Clearance of glutamate inside the synapse and beyond

被引:250
作者
Bergles, DE [1 ]
Diamond, JS [1 ]
Jahr, CE [1 ]
机构
[1] Oregon Hlth & Sci Univ, Vollum Inst, Portland, OR 97201 USA
关键词
D O I
10.1016/S0959-4388(99)80043-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The heated debate over the level of postsynaptic receptor occupancy by transmitter has not been extinguished - indeed, new evidence is fanning the flames, Recent experiments using two-photon microscopy suggest that the concentration of glutamate in the synaptic cleft does not attain levels previously suggested. In contrast, recordings from glial cells and studies of extrasynaptic receptor activation indicate that significant quantities of glutamate escape from the cleft following exocytosis. Determining the amount of glutamate efflux from the synaptic cleft and the distance it diffuses is critical to issues of synaptic specificity and the induction of synaptic plasticity.
引用
收藏
页码:293 / 298
页数:6
相关论文
共 63 条
[1]   TRANSMITTER RELEASE FROM SYNAPSES - DOES A PREASSEMBLED FUSION PORE INITIATE EXOCYTOSIS [J].
ALMERS, W ;
TSE, FW .
NEURON, 1990, 4 (06) :813-818
[2]   Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance [J].
Arriza, JL ;
Eliasof, S ;
Kavanaugh, MP ;
Amara, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) :4155-4160
[3]   Extrasynaptic glutamate spillover in the hippocampus: Dependence on temperature and the role of active glutamate uptake [J].
Asztely, F ;
Erdemli, G ;
Kullmann, DM .
NEURON, 1997, 18 (02) :281-293
[4]   PROLONGED PRESENCE OF GLUTAMATE DURING EXCITATORY SYNAPTIC TRANSMISSION TO CEREBELLAR PURKINJE-CELLS [J].
BARBOUR, B ;
KELLER, BU ;
LLANO, I ;
MARTY, A .
NEURON, 1994, 12 (06) :1331-1343
[5]  
Barbour B, 1997, TRENDS NEUROSCI, V20, P377
[6]   Glutamate transporter currents in Bergmann glial cells follow the time course of extrasynaptic glutamate [J].
Bergles, DE ;
Dzubay, JA ;
Jahr, CE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) :14821-14825
[7]   Synaptic activation of glutamate transporters in hippocampal astrocytes [J].
Bergles, DE ;
Jahr, CE .
NEURON, 1997, 19 (06) :1297-1308
[8]  
BERGLES DE, 1994, NEURON, V13, P713
[9]   REAL-TIME MEASUREMENT OF TRANSMITTER RELEASE FROM SINGLE SYNAPTIC VESICLES [J].
BRUNS, D ;
JAHN, R .
NATURE, 1995, 377 (6544) :62-65
[10]   GLUTAMATE TRANSPORTERS IN GLIAL PLASMA-MEMBRANES - HIGHLY DIFFERENTIATED LOCALIZATIONS REVEALED BY QUANTITATIVE ULTRASTRUCTURAL IMMUNOCYTOCHEMISTRY [J].
CHAUDHRY, FA ;
LEHRE, KP ;
CAMPAGNE, MV ;
OTTERSEN, OP ;
DANBOLT, NC ;
STORMMATHISEN, J .
NEURON, 1995, 15 (03) :711-720