Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4

被引：130

作者：

Kim, Hyung Sook ^{[1
,2
]}

Kim, Yeon Jin ^{[1
,2
]}

Lee, Hong Kyung ^{[1
,2
]}

Ryu, Hwa Sun ^{[1
,2
]}

Kim, Ji Sung ^{[1
,2
]}

Yoon, Mi Jung ^{[1
,2
]}

Kang, Jong Soon ^{[3
]}

Hong, Jin Tae ^{[1
,2
]}

Kim, Youngsoo ^{[1
,2
]}

Han, Sang-Bae ^{[1
,2
]}

机构：

[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungbuk, South Korea

[2] Chungbuk Natl Univ, Med Res Ctr CICT, Cheongju 361763, Chungbuk, South Korea

[3] Korea Res Inst Biosci & Biotechnol, Ochang 363883, Chungbuk, South Korea

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2012年 / 50卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

Paecilomyces cicadae; Polysaccharide; Macrophage; TLR4; NF-kappa B; MAPK; NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; DENDRITIC CELL MATURATION; PLATYCODON-GRANDIFLORUM; GANODERMA-LUCIDUM; CORDYCEPS-MILITARIS; RAW264.7; CELLS; PROTEIN-KINASE; EXPRESSION; RADIX;

D O I：

10.1016/j.fct.2012.05.051

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Paecilomyces cicadae have been reported to have immunomodulatory properties. In this study, we investigated the effect of polysaccharide (PCP) isolated from P. cicadae on the macrophages. PCP increased the production of nitric oxide (NO) and the gene expression of IL-1 beta, IL-6, and TNF-alpha in RAW 264.7 cells. To investigate the membrane receptor, we examined the effect of PCP on primary macrophages isolated from wild type C3H/HeN and C3H/HeJ mice having mutant-TLR4. PCP induced NO production and cytokine gene expression in macrophages from C3H/HeN, but not from tlr4-mutated C3H/HeJ mice, which suggests that TLR4 is the membrane receptor for PCP. PCP induced the phosphorylation of ERK, JNK, and p38, and the nuclear translocation of NF-kappa B p50/p65, which are the main signaling molecules downstream from TLR4. Among them, p38 and NF-kappa B signaling played a crucial role in PCP-induced NO production by macrophages. These results indicate that PCP activates macrophages through the TLR4 signaling pathway. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：3190 / 3197

页数：8

共 40 条

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