Early diagnosis of bladder cancer through the detection of urinary tyrosine-phosphorylated proteins

被引：13

作者：

Khadjavi, A. ^{[1
]}

Mannu, F. ^{[2
]}

Destefanis, P. ^{[3
]}

Sacerdote, C. ^{[4
,5
]}

Battaglia, A. ^{[3
]}

Allasia, M. ^{[3
]}

Fontana, D. ^{[3
]}

Frea, B. ^{[3
]}

Polidoro, S. ^{[6
,7
]}

Fiorito, G. ^{[6
,7
]}

Matullo, G. ^{[6
,7
]}

Pantaleo, A. ^{[8
]}

Notarpietro, A. ^{[9
]}

Prato, M. ^{[1
]}

Castagno, F. ^{[10
]}

Vineis, P. ^{[11
]}

Gontero, P. ^{[3
]}

Giribaldi, G. ^{[9
]}

Turrini, F. ^{[9
]}

机构：

[1] Univ Turin, Dept Neurosci, I-10126 Turin, Italy

[2] Nurex Srl, Sassari, Italy

[3] Univ Turin, Dept Surg Sci, Hosp San Giovanni Battista, Urol Clin Citta Salute & Sci Torino, I-10126 Turin, Italy

[4] Univ Turin, Dept Med Sci, Unit Canc Epidemiol, I-10126 Turin, Italy

[5] Ctr Canc Epidemiol & Prevent CPO Piemonte, Turin, Italy

[6] Univ Turin, HuGeF Human Genet Fdn, I-10126 Turin, Italy

[7] Univ Turin, Dept Med Sci, I-10126 Turin, Italy

[8] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy

[9] Univ Turin, Sch Med, Dept Oncol, I-10126 Turin, Italy

[10] Hosp San Giovanni Battista, Blood Ctr AO Citta Salute & Sci Torino, Turin, Italy

[11] Univ London Imperial Coll Sci Technol & Med, Sch Publ Hlth, MRC PHE Ctr Environm & Hlth, London, England

来源：

BRITISH JOURNAL OF CANCER | 2015年 / 113卷 / 03期

关键词：

bladder cancer; urinary tyrosine-phosphorylated proteins; urinary tumour markers; diagnostic assay; 5-AMINOLEVULINIC ACID; MARKERS; IDENTIFICATION; FLUORESCENCE; ASSAY;

D O I：

10.1038/bjc.2015.232

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: A noninvasive, highly sensitive and specific urine test is needed for bladder cancer (BC) diagnosis and surveillance in addition to the invasive cystoscopy. We previously described the diagnostic effectiveness of urinary tyrosine-phosphorylated proteins (UPY) and a new assay (UPY-A) for their measurement in a pilot study. The aim of this work was to evaluate the performances of the UPY-A using an independent cohort of 262 subjects. Methods: Urinary tyrosine-phosphorylated proteins were measured by UPY-A test. The area under ROC curve, cutoff, sensitivity, specificity and predictive values of UPY-A were determined. The association of UPY levels with tumour staging, grading, recurrence and progression risk was analysed by Kruskal-Wallis and Wilcoxon's test. To test the probability to be a case if positive at the UPY-A, a logistic test adjusted for possible confounding factor was used. Results: Results showed a significant difference of UPY levels between patients with BC vs healthy controls. For the best cutoff value, 261.26 Standard Units (SU), the sensitivity of the assay was 80.43% and the specificity was 78.82%. A statistically significant difference was found in the levels of UPY at different BC stages and grades between Ta and T1 and with different risk of recurrence and progression. A statistically significant increased risk for BC at UPY-A >= 261.26 SU was observed. Conclusions: The present study supplies important information on the diagnostic characteristics of UPY-A revealing remarkable performances for early stages and allowing its potential use for different applications encompassing the screening of high-risk subjects, primary diagnosis and posttreatment surveillance.

引用

页码：469 / 475

页数：7

共 28 条

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