Protein synthesis inhibitor-mediated stability of adenomatous polyposis coli mRNA levels in HCT-116 colon cancer cells

被引：0
作者：
Jaiswal, AS
Narayan, S
机构：
[1] Univ Texas, Med Branch, Sealy Ctr Oncol & Hematol, Galveston, TX 77555 USA
[2] Univ Texas, Med Branch, Dept Human Biol Chem & Genet, Galveston, TX 77555 USA
来源：
INTERNATIONAL JOURNAL OF ONCOLOGY | 1999年 / 14卷 / 06期
关键词：
colon cancer; adenomatous polyposis coli (APC) gene expression; cycloheximide; mRNA stability; transcription;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We examined the effect of a protein synthesis inhibitor, cycloheximide (CHX) on the adenomatous polyposis coli (APC) mRNA levels in HCT-116 colon cancer cell line. The HCT-116 cells were treated with different concentrations of CHX for 15 h. APC, p53 and beta-actin mRNA levels were determined by Northern blotting. Results showed that APC and beta-actin mRNA levels were significantly increased in a dose-dependent manner after CHX treatment. The p53 mRNA levels were moderately increased. The increase in APC mRNA levels after CHX treatment was due to increase in its stability instead of transcription. These results provide a model for CHX-induced APC mRNA stabilization and its implication in cell cycle arrest and cell survival studies.
引用
收藏
页码：1045 / 1048
页数：4
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