Protein synthesis inhibitor-mediated stability of adenomatous polyposis coli mRNA levels in HCT-116 colon cancer cells

被引：0
作者：
Jaiswal, AS
Narayan, S
机构：
[1] Univ Texas, Med Branch, Sealy Ctr Oncol & Hematol, Galveston, TX 77555 USA
[2] Univ Texas, Med Branch, Dept Human Biol Chem & Genet, Galveston, TX 77555 USA
来源：
INTERNATIONAL JOURNAL OF ONCOLOGY | 1999年 / 14卷 / 06期
关键词：
colon cancer; adenomatous polyposis coli (APC) gene expression; cycloheximide; mRNA stability; transcription;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We examined the effect of a protein synthesis inhibitor, cycloheximide (CHX) on the adenomatous polyposis coli (APC) mRNA levels in HCT-116 colon cancer cell line. The HCT-116 cells were treated with different concentrations of CHX for 15 h. APC, p53 and beta-actin mRNA levels were determined by Northern blotting. Results showed that APC and beta-actin mRNA levels were significantly increased in a dose-dependent manner after CHX treatment. The p53 mRNA levels were moderately increased. The increase in APC mRNA levels after CHX treatment was due to increase in its stability instead of transcription. These results provide a model for CHX-induced APC mRNA stabilization and its implication in cell cycle arrest and cell survival studies.
引用
收藏
页码：1045 / 1048
页数：4
相关论文
共 33 条
[1]   COMPLEXITY OF THE EARLY GENETIC RESPONSE TO GROWTH-FACTORS IN MOUSE FIBROBLASTS [J].
ALMENDRAL, JM ;
SOMMER, D ;
MACDONALDBRAVO, H ;
BURCKHARDT, J ;
PERERA, J ;
BRAVO, R .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (05) :2140-2148
[2]   THE TUMOR-SUPPRESSOR GENE-PRODUCT APC BLOCKS CELL-CYCLE PROGRESSION FROM G(0)/G(1) TO S-PHASE [J].
BAEG, GH ;
MATSUMINE, A ;
KURODA, T ;
BHATTACHARJEE, RN ;
MIYASHIRO, I ;
TOYOSHIMA, K ;
AKIYAMA, T .
EMBO JOURNAL, 1995, 14 (22) :5618-5625
[3]   NH2-terminal deletion of beta-catenin results in stable colocalization of mutant beta-catenin with adenomatous polyposis coli protein and altered MDCK cell adhesion [J].
Barth, AIM ;
Pollack, AL ;
Altschuler, Y ;
Mostov, KE ;
Nelson, WJ .
JOURNAL OF CELL BIOLOGY, 1997, 136 (03) :693-706
[4]   THE POLY(A)-POLY(A)-BINDING PROTEIN COMPLEX IS A MAJOR DETERMINANT OF MESSENGER-RNA STABILITY INVITRO [J].
BERNSTEIN, P ;
PELTZ, SW ;
ROSS, J .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (02) :659-670
[5]   UP-REGULATION OF BETA(1)-ADRENOCEPTOR MESSENGER-RIBONUCLEIC-ACID IN THE RAT PINEAL-GLAND - NOCTURNALLY, THROUGH A BETA-ADRENOCEPTOR-LINKED MECHANISM, AND IN-VITRO, THROUGH A NOVEL POSTTRANSCRIPTIONAL MECHANISM ACTIVATED BY SPECIFIC PROTEIN-SYNTHESIS INHIBITORS [J].
CARTER, DA .
ENDOCRINOLOGY, 1993, 133 (05) :2263-2268
[6]   SPECIFIC STIMULATION OF ACTIN GENE-TRANSCRIPTION BY EPIDERMAL GROWTH-FACTOR AND CYCLOHEXIMIDE [J].
ELDER, PK ;
SCHMIDT, LJ ;
ONO, T ;
GETZ, MJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (23) :7476-7480
[7]   REGULATION OF C-FOS GENE-EXPRESSION IN HAMSTER FIBROBLASTS - INITIATION AND ELONGATION OF TRANSCRIPTION AND MESSENGER-RNA DEGRADATION [J].
FORT, P ;
RECH, J ;
VIE, A ;
PIECHACZYK, M ;
BONNIEU, A ;
JEANTEUR, P ;
BLANCHARD, JM .
NUCLEIC ACIDS RESEARCH, 1987, 15 (14) :5657-5667
[8]   PROGRESSION OF COLORECTAL-CANCER IS ASSOCIATED WITH MULTIPLE TUMOR SUPPRESSOR GENE DEFECTS BUT INHIBITION OF TUMORIGENICITY IS ACCOMPLISHED BY CORRECTION OF ANY SINGLE DEFECT VIA CHROMOSOME TRANSFER [J].
GOYETTE, MC ;
CHO, K ;
FASCHING, CL ;
LEVY, DB ;
KINZLER, KW ;
PARASKEVA, C ;
VOGELSTEIN, B ;
STANBRIDGE, EJ .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (03) :1387-1395
[9]  
GRODEN J, 1995, CANCER RES, V55, P1531
[10]   EXPRESSION OF THE APC GENE AFTER TRANSFECTION INTO A COLONIC-CANCER CELL-LINE [J].
HARGEST, R ;
WILLIAMSON, R .
GUT, 1995, 37 (06) :826-829
1234