Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nontuberculous Mycobacteria from Clinical Isolates

被引：38

作者：

Rodriguez-Sanchez, Belen ^{[1
,2
,3
]}

Jesus Ruiz-Serrano, Mara ^{[1
,2
,3
]}

Marin, Mercedes ^{[1
,2
,3
,4
]}

Lopez Roa, Paula ^{[1
]}

Rodriguez-Creixems, Marta ^{[1
]}

Bouza, Emilio ^{[1
,2
,3
,4
]}

机构：

[1] Univ Gregorio Maranon, Gen Hosp, Clin Microbiol & Infect Dis Dept, Madrid, Spain

[2] CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain

[3] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain

[4] Univ Complutense Madrid, Sch Med, Dept Med, Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 08期

关键词：

RAPID IDENTIFICATION; CULTURE-MEDIA;

D O I：

10.1128/JCM.01380-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of nontuberculous mycobacterial (NTM) isolates was evaluated in this study. Overall, 125 NTM isolates were analyzed by MALDI-TOF and GenoType CM/AS. Identification by 16S rRNA/hsp65 sequencing was considered the gold standard. Agreements between MALDI-TOF and GenoType CM/AS with the reference method were, respectively, 94.4% and 84.0%. In 17 cases (13.6%), results provided by GenoType and MALDI-TOF were discordant; however, the reference method agreed with MALDI-TOF in 16/17 cases (94.1%; P = 0.002).

引用

页码：2737 / 2740

页数：4

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