Mammalian DNMTs in the male germ line DNA of Drosophila

被引：0

作者：

Weyrich, Alexandra ^{[1
,5
]}

Tang, Xiaojing ^{[1
]}

Xu, Guoliang ^{[4
]}

Schrattenholz, Andre ^{[3
]}

Hunzinger, Christian ^{[3
]}

Hennig, Wolfgang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, MPG Guest Lab, DAAD Lab, Shanghai 200031, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Shanghai 200031, Peoples R China

[3] ProteoSys, D-55149 Mainz, Germany

[4] Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

[5] Johannes Gutenberg Univ Mainz, Inst Genet, Mainz, Germany

来源：

BIOCHEMISTRY AND CELL BIOLOGY | 2008年 / 86卷 / 05期

关键词：

Drosophila; DNA methylation; male germ line; spermatogenesis; DNMT3;

D O I：

10.1139/O08-096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is controversial whether DNA methylation plays a functional role in Drosophila. We have studied testis DNA of Drosophila melanogaster Meigen, 1830 with antisera against 5-methylcytosine (5mC) and found no evidence for the presence of significant amounts of 5mC. Reactions occur only with I of 3 5mC antisera, but they are restricted to nuclear regions without detectable amounts of DNA. The antisera apparently cross-react with other nuclear components. If the murine de novo DNA methyltransferases, DNMT3A and DNMT3B, are expressed under the control of the spermatocyte-specific beta 2-tubulin promoter in testes, DNA methylation is not increased and no effects on the fertility of the fly are seen. DNA methylation has, therefore, no functional relevance in the male germ line of Drosophila.

引用

页码：380 / 385

页数：6

共 29 条

[1] CpG islands as genomic footprints of promoters that are associated with replication origins [J].

Antequera, F ;

Bird, A .

CURRENT BIOLOGY, 1999, 9 (17) :R661-R667

[2] Transposon silencing and imprint establishment in mammalian germ cells [J].

Bestor, TH ;

Bourc'his, D .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2004, 69 :381-387

[3]

Brennecke J, 2007, CELL, V128, P1089, DOI 10.1016/j.cell.2007.01.043

[4] Chromatin targeting of de novo DNA methyltransferases by the PWWP domain [J].

Ge, YZ ;

Pu, MT ;

Gowher, H ;

Wu, HP ;

Ding, JP ;

Jeltsch, A ;

Xu, GL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (24) :25447-25454

[5] Methylation of tRNAAsP by the DNA methyltransferase homolog Dnmt2 [J].

Goll, MG ;

Kirpekar, F ;

Maggert, KA ;

Yoder, JA ;

Hsieh, CL ;

Zhang, XY ;

Golic, KG ;

Jacobsen, SE ;

Bestor, TH .

SCIENCE, 2006, 311 (5759) :395-398

[6] DNA of Drosophila melanogaster contains 5-methylcytosine [J].

Gowher, H ;

Leismann, O ;

Jeltsch, A .

EMBO JOURNAL, 2000, 19 (24) :6918-6923

[7]

GROND CJ, 1984, THESIS U NIJMEGEN NI

[8]

Grunau C, 2001, Nucleic Acids Res, V29, pE65, DOI 10.1093/nar/29.13.e65

[9]

Hennig W, 1996, INT J DEV BIOL, V40, P167

[10]

Hennig W, 1990, Int Rev Cytol, V123, P129, DOI 10.1016/S0074-7696(08)60673-7

← 1 2 3 →