Molecular detection and identification of phytoplasma associated with pepper witches' broom in China

被引：8

作者：

Li, Zheng-Nan ^{[1
,2
]}

Zhang, Lei ^{[3
]}

Song, Jia-Gui ^{[1
,2
]}

Wu, Yun-Feng ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Minist Agr, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Key Lab Crop Pest Integrated Pest Management Loes, Minist Agr, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China

[3] Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China

来源：

PHYTOPARASITICA | 2013年 / 41卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Cicadella viridis; Insect vector; 16S rRNA gene; Tuf gene; ASTER YELLOWS PHYTOPLASMA; 16S RIBOSOMAL-RNA; 1ST REPORT; CLASSIFICATION; ROSE;

D O I：

10.1007/s12600-013-0304-2

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Pepper witches' broom (PWB) disease was observed in a field in Yangling, Shaanxi Province, China. The result of mechanical inoculation test for this disease was negative. Phytoplasma-like bodies were observed in ultrathin sections of petiole tissues of symptomatic samples. 16S rRNA gene and tuf gene of phytoplasma were amplified from the total DNA of symptomatic samples. Phylogeny analysis of the 16S rRNA gene and tuf gene suggested that the pepper witches' broom associated phytoplasma belongs to the subgroup 16SrI-B, which was confirmed by the RFLP analysis of the 16S rRNA gene. The phytoplasma subgroup 16SrI-B was also detected in the vector Cicadella viridis trapped from the infected field. To our knowledge, this is the first report of 16SrI-B phytoplasma causing pepper witches' broom in China.

引用

页码：429 / 434

页数：6

共 20 条

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