The proteome of mouse vestibular hair bundles over development

被引：33
作者：
Krey, Jocelyn F. [1 ,2 ]
Sherman, Nicholas E. [3 ]
Jeffery, Erin D. [3 ]
Choi, Dongseok [4 ]
Barr-Gillespie, Peter G. [1 ,2 ]
机构：
[1] Oregon Hlth & Sci Univ, Oregon Hearing Res Ctr, Portland, OR 97239 USA
[2] Oregon Hlth & Sci Univ, Vollum Inst, Portland, OR 97239 USA
[3] Univ Virginia, WM Keck Biomed Mass Spectrometry Lab, Charlottesville, VA 22908 USA
[4] Oregon Hlth & Sci Univ, Dept Publ Hlth & Prevent Med, Portland, OR 97239 USA
来源：
SCIENTIFIC DATA | 2015年 / 2卷
关键词：
D O I：
10.1038/sdata.2015.47
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development.
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页数：10
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共 21 条
  • [1] Developing top down proteomics to maximize proteome and sequence coverage from cells and tissues
    Ahlf, Dorothy R.
    Thomas, Paul M.
    Kelleher, Neil L.
    [J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2013, 17 (05) : 787 - 794
  • [2] Over Half the Hair Cells in the Mouse Utricle First Appear After Birth, with Significant Numbers Originating from Early Postnatal Mitotic Production in Peripheral and Striolar Growth Zones
    Burns, Joseph C.
    On, Doan
    Baker, Wendy
    Collado, M. Sol
    Corwin, Jeffrey T.
    [J]. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY, 2012, 13 (05): : 609 - 627
  • [3] Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment
    Cox, Juergen
    Neuhauser, Nadin
    Michalski, Annette
    Scheltema, Richard A.
    Olsen, Jesper V.
    Mann, Matthias
    [J]. JOURNAL OF PROTEOME RESEARCH, 2011, 10 (04) : 1794 - 1805
  • [4] MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification
    Cox, Juergen
    Mann, Matthias
    [J]. NATURE BIOTECHNOLOGY, 2008, 26 (12) : 1367 - 1372
  • [5] The adult mouse utricle as an in vitro preparation for studies of ototoxic-drug-induced sensory hair cell death
    Cunningham, Lisa L.
    [J]. BRAIN RESEARCH, 2006, 1091 : 277 - 281
  • [6] Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia
    Dumont, RA
    Zhao, YD
    Holt, JR
    Bähler, M
    Gillespie, PG
    [J]. JARO-JOURNAL OF THE ASSOCIATION FOR RESEARCH IN OTOLARYNGOLOGY, 2002, 3 (04): : 375 - 389
  • [7] A Short Splice Form of Xin-Actin Binding Repeat Containing 2 (XIRP2) Lacking the Xin Repeats Is Required for Maintenance of Stereocilia Morphology and Hearing Function
    Francis, Shimon P.
    Krey, Jocelyn F.
    Krystofiak, Evan S.
    Cui, Runjia
    Nanda, Sonali
    Xu, Wenhao
    Kachar, Bechara
    Barr-Gillespie, Peter G.
    Shin, Jung-Bum
    [J]. JOURNAL OF NEUROSCIENCE, 2015, 35 (05) : 1999 - 2014
  • [8] HIGH-PURITY ISOLATION OF BULLFROG HAIR BUNDLES AND SUBCELLULAR AND TOPOLOGICAL LOCALIZATION OF CONSTITUENT PROTEINS
    GILLESPIE, PG
    HUDSPETH, AJ
    [J]. JOURNAL OF CELL BIOLOGY, 1991, 112 (04) : 625 - 640
  • [9] Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis
    Gillet, Ludovic C.
    Navarro, Pedro
    Tate, Stephen
    Roest, Hannes
    Selevsek, Nathalie
    Reiter, Lukas
    Bonner, Ron
    Aebersold, Ruedi
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2012, 11 (06)
  • [10] Shaping sound in space: the regulation of inner ear patterning
    Groves, Andrew K.
    Fekete, Donna M.
    [J]. DEVELOPMENT, 2012, 139 (02): : 245 - 257
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