Urinary Exosomal MicroRNAs in Incipient Diabetic Nephropathy

被引：279

作者：

Barutta, Federica ^{[1
]}

Tricarico, Marinella ^{[1
]}

Corbelli, Alessandro ^{[2
,3
,4
]}

Annaratone, Laura ^{[5
]}

Pinach, Silvia ^{[1
]}

Grimaldi, Serena ^{[1
]}

Bruno, Graziella ^{[1
]}

Cimino, Daniela ^{[6
]}

Taverna, Daniela ^{[6
]}

Deregibus, Maria Chiara ^{[7
]}

Rastaldi, Maria Pia

Perin, Paolo Cavallo ^{[1
]}

Gruden, Gabriella ^{[1
]}

机构：

[1] Univ Turin, Dept Med Sci, Diabet Nephropathy Lab, Turin, Italy

[2] Osped Maggiore Policlin, Fdn IRCCS, Renal Res Lab, Milan, Italy

[3] Fdn DAmico Ric Malattie Renali, Milan, Italy

[4] Milano Bicocca Univ, MIA Consortium Image Anal, Milan, Italy

[5] Univ Turin, Dept Biomed Sci & Human Oncol, I-10124 Turin, Italy

[6] Univ Turin, Ctr Mol Biotechnol, Turin, Italy

[7] Univ Turin, Dept Med Sci, Lab Renal & Vasc Pathophysiol, Turin, Italy

来源：

PLOS ONE | 2013年 / 8卷 / 11期

关键词：

KIDNEY GLOMERULI; EXPRESSION; CELLS; MICROALBUMINURIA; TGF-BETA-1; MIR-145; PLASMA;

D O I：

10.1371/journal.pone.0073798

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNAs (miRNAs), a class of small non-protein-encoding RNAs, regulate gene expression via suppression of target mRNAs. MiRNAs are present in body fluids in a remarkable stable form as packaged in microvesicles of endocytic origin, named exosomes. In the present study, we have assessed miRNA expression in urinary exosomes from type 1 diabetic patients with and without incipient diabetic nephropathy. Results showed that miR-130a and miR-145 were enriched, while miR-155 and miR-424 reduced in urinary exosomes from patients with microalbuminuria. Similarly, in an animal model of early experimental diabetic nephropathy, urinary exosomal miR-145 levels were increased and this was paralleled by miR-145 overexpression within the glomeruli. Exposure of cultured mesangial cells to high glucose increased miR-145 content in both mesangial cells and mesangial cells-derived exosomes, providing a potential mechanism for diabetes-induced miR-145 overexpression. In conclusion, urinary exosomal miRNA content is altered in type 1 diabetic patients with incipient diabetic nephropathy and miR-145 may represent a novel candidate biomarker/player in the complication.

引用

页数：8

共 39 条

[1] Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes [J].