Detection of Oropouche virus segment S in patients and in Culex quinquefasciatus in the state of Mato Grosso, Brazil

被引：29

作者：

Cardoso, Belgath Fernandes ^{[1
]}

Serra, Otacilia Pereira ^{[1
]}

da Silva Heinen, Leticia Borges ^{[1
]}

Zuchi, Nayara ^{[1
]}

de Souza, Victor Costa ^{[2
]}

Naveca, Felipe Gomes ^{[2
]}

Mendes dos Santos, Marcelo Adriano ^{[3
]}

Slhessarenko, Renata Dezengrini ^{[1
]}

机构：

[1] Univ Fed Mato Grosso, Fac Med, Programa Posgrad Ciencias Saude, Cuiaba, MT, Brazil

[2] Fundacao Oswaldo Cruz, Inst Leonidas & Maria Deane, Manaus, AM, Brazil

[3] Cent Lab, Secretaria Estado Saude, Cuiaba, MT, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2015年 / 110卷 / 06期

关键词：

OROV; Orthobunyavirus; Mato Grosso; mosquitoes; phylogenetic analysis; LOUIS ENCEPHALITIS-VIRUS; ORTHOBUNYAVIRUS; IDENTIFICATION; EPIDEMIOLOGY; CULICIDAE; INFECTION; SANTAREM; AREA; TRANSMISSION; REASSORTANT;

D O I：

10.1590/0074-02760150123

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This study aimed to investigate the circulation of Orthobunyavirus species in the state of Mato Grosso (MT) Brazil. During a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female Culex quinquefasciatus captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment S of the Simbu serogroup followed by nucleotide sequencing and virus isolation in Vero cells. Patients (5/529; 0.9%) from Cuiaba (n = 3), Varzea Grande (n = 1) and Nova Mutum (n = 1) municipalities were positive for the S segment of Oropouche virus (OROV). Additionally, eight/387 Cx. quinquefasciatus pools were positive for the segment, with a minimum infection rate of 2.3. Phylogenetic analysis indicated that all the samples belong to the subgenotype Ia, presenting high homology with OROV strains obtained from humans and animals in the Brazilian Amazon. The present paper reports the first detection of an Orthobunyavirus, possibly OROV, in patients and in Cx. quinquefasciatus mosquitoes in MT. This finding reinforces the notion that arboviruses frequently reported in the Amazon Region circulate sporadically in MT during dengue outbreaks.

引用

页码：745 / 754

页数：10

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