Complete genome sequence analysis of an American isolate of Grapevine virus E

被引:12
|
作者
Alabi, Olufemi J. [1 ]
Poojari, Sudarsana [1 ]
Sarver, Kara [2 ]
Martin, Robert R. [2 ]
Naidu, Rayapati A. [1 ]
机构
[1] Washington State Univ, Ctr Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA
[2] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA
基金
美国食品与农业研究所; 美国农业部;
关键词
Grapevine virus E; Vitis sp; Vitiviruses; Betaflexiviridae; RNA HELICASE;
D O I
10.1007/s11262-012-0872-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The complete genome sequence of Grapevine virus E (GVE) collected from a red-berried wine grape cultivar (Cabernet Sauvignon) in Washington State was determined. The 7,568 nucleotide long genome of GVE is more similar in sequence identity with a GVE isolate from a wine grape cv. Shiraz from South Africa when compared with an isolate from "Aki Queen" grape from Japan. Like GVE isolates from South Africa and Japan, the Washington isolate encodes five open reading frames (ORFs) and the overall genome organization is identical among these isolates. In addition to AlkB domain, a DExD domain, belonging to the DEAD-like helicases superfamily, was present upstream of the helicase domain in the replicase ORF of the virus.
引用
收藏
页码:563 / 566
页数:4
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