The Osteogenic Response of Undifferentiated Human Adipose-Derived Stem Cells under Mechanical Stimulation

被引:14
作者
Du, Hong-ming [2 ]
Zheng, Xiao-hui [1 ]
Wang, Li-ya [2 ]
Tang, Wei [1 ]
Liu, Lei [1 ]
Jing, Wei [1 ]
Lin, Yun-feng [2 ]
Tian, Wei-dong [1 ]
Long, Jie [1 ,2 ]
机构
[1] Sichuan Univ, W China Coll Stomatol, Dept Oral & Maxillofacial Surg, Chengdu 610041, Peoples R China
[2] Sichuan Univ, W China Coll Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China
关键词
Human adipose-derived stem cells; Stretch loading; Osteogenic differentiation; Chemical stimulation; OSCILLATORY FLUID-FLOW; CYCLIC TENSILE STRAIN; MARROW STROMAL CELLS; BONE-MARROW; CHONDROGENIC DIFFERENTIATION; OSTEOBLAST DIFFERENTIATION; IN-VITRO; TISSUE; PROLIFERATION; EXPRESSION;
D O I
10.1159/000335905
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The purpose of this study was to investigate the osteogenic response of human adipose-derived stem cells (hASCs) under mechanical and/or chemical stimulation. hASCs were divided into three groups. In group A, the cells were cultured without any stimulation, in group B, the cells were induced with chemical stimulation, and in group C, the cells were induced with a combination of chemical stimulation and stretch loading. Stretch loading and chemical stimulation were applied using a four-point bending apparatus (0.5 Hz, 2,000 mu epsilon, 2 h/day) and osteogenic differentiation medium, respectively. At the 1st, 2nd, 3rd, 5th and 7th day following initiation of stretch loading, we detected alkaline phosphatase activity, mRNA expression (RUNX2, ALPL, osteonectin, osteopontin and type I collagen) and protein expression (RUNX2 and osteopontin) by colorimetric assay, real-time PCR and Western blot methods, respectively. Alkaline phosphatase activity, mRNA expression and protein expression all increased in groups B and C along with the culture time, but were observed to be downregulated by the 7th day in group C (p < 0.05). Compared to group A, most of the above markers were significantly higher in groups B and C (p < 0.05). All of the above markers in group C were higher than those in group B before the 5th day (p < 0.05), except at the 1st day. These results indicated that stretch loading promoted osteogenic differentiation of hASCs and that the combination of mechanical and chemical stimulation could enhance the osteogenic capability up to the 5th day relative to chemical stimulation alone. Copyright (c) 2012 S. Karger AG, Basel
引用
收藏
页码:313 / 324
页数:12
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