Mechanism of Synergistic Inhibition of Listeria monocytogenes Growth by Lactic Acid, Monolaurin, and Nisin

被引：31

作者：

Tokarskyy, Oleksandr ^{[2
]}

Marshall, Douglas L. ^{[1
]}

机构：

[1] Univ No Colorado, Coll Nat & Hlth Sci, Greeley, CO 80639 USA

[2] Mississippi State Univ, Mississippi Agr & Forestry Expt Stn, Dept Food Sci Nutr & Hlth Promot, Mississippi State, MS 39762 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 23期

关键词：

D O I：

10.1128/AEM.01292-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The combined lactic acid, monolaurin, and nisin effects on time-to-detection (optical density at 600 nm) extension were greater (P < 0.05) than any single or paired combination effect, which demonstrates a synergistic interaction among the antimicrobials. Monolaurin exposure caused C12:0 cell membrane incorporation. Lactic acid caused increased monolaurin C12:0 membrane incorporation, while nisin had no influence. We postulate that lactic acid-enhanced monolaurin C12:0 incorporation into the cell membrane increased membrane fluidity resulting in increased nisin activity.

引用

页码：7126 / 7129

页数：4

共 18 条

[1] INHIBITORY-ACTION OF NISIN AGAINST LISTERIA-MONOCYTOGENES [J].

BENKERROUM, N ;

SANDINE, WE .

JOURNAL OF DAIRY SCIENCE, 1988, 71 (12) :3237-3245

[2] Nisin Z, mutant nisin Z and lacticin 481 interactions with anionic lipids correlate with antimicrobial activity - A monolayer study [J].

Demel, RA ;

Peelen, T ;

Siezen, RJ ;

deKruijff, B ;

Kuipers, OP .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 235 (1-2) :267-274

[3]

Doores S, 2005, FOOD SCI, V143, P91

[4] Development of a method to quantify in vitro the synergistic activity of "natural" antimicrobials [J].

Dufour, M ;

Simmonds, RS ;

Bremer, PJ .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 85 (03) :249-258

[5] INFLUENCE OF ALTERED FATTY-ACID COMPOSITION ON RESISTANCE OF LISTERIA-MONOCYTOGENES TO ANTIMICROBIALS [J].

JUNEJA, VK ;

DAVIDSON, PM .

JOURNAL OF FOOD PROTECTION, 1993, 56 (04) :302-305

[6]

Kabara JJ, 2005, FOOD SCI, V143, P327

[7] Temperature- and surfactant-induced membrane modifications that alter Listeria monocytogenes nisin sensitivity by different mechanisms [J].

Li, J ;

Chikindas, ML ;

Ludescher, RD ;

Montville, TJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) :5904-5910

[8] An inhibitory synergistic effect of a nisin-monolaurin combination on Bacillus sp vegetative cells in milk [J].

Mansour, M ;

Millière, JB .

FOOD MICROBIOLOGY, 2001, 18 (01) :87-94

[9] Nisin induces changes in membrane fatty acid composition of Listeria monocytogenes nisin-resistant strains at 10 degrees C and 30 degrees C [J].

Mazzotta, AS ;

Montville, TJ .

JOURNAL OF APPLIED MICROBIOLOGY, 1997, 82 (01) :32-38

[10] Synergy between nisin and select lactates against Listeria monocytogenes is due to the metal cations [J].

McEntire, JC ;

Montville, TJ ;

Chikindas, ML .

JOURNAL OF FOOD PROTECTION, 2003, 66 (09) :1631-1636

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