Role of diacylglycerol in PKD recruitment to the TGN and protein transport to the plasma membrane

被引：336

作者：

Baron, CL ^{[1
]}

Malhotra, V ^{[1
]}

机构：

[1] Univ Calif San Diego, Div Biol, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

来源：

SCIENCE | 2002年 / 295卷 / 5553期

关键词：

D O I：

10.1126/science.1066759

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein kinase D (PKD) is a cytosolic serine-threonine kinase that binds to the trans-Golgi network (TGN) and regulates the fission of transport carriers specifically destined to the cell surface. PKD was found to bind diacylglycerol (DAG), and this binding was necessary for its recruitment to the TGN. Reducing cellular levels of DAG inhibited PKD recruitment and blocked protein transport from the TGN to the cell surface. Thus, a DAG-dependent, PKD-mediated signaling regulates the formation of transport carriers from the TGN in mammalian cells.

引用

页码：325 / 328

页数：4

共 23 条

[1]

BARON C, UNPUB

[2]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[3] RAPID ANTAGONISTIC ACTIONS OF NORADRENALINE AND INSULIN ON RAT ADIPOCYTE PHOSPHATIDATE PHOSPHOHYDROLASE ACTIVITY [J].

CHENG, CHK ;

SAGGERSON, ED .

FEBS LETTERS, 1978, 93 (01) :120-124

[4] DIACYLGLYCEROLS RELEASE LH - STRUCTURE-ACTIVITY RELATIONS REVEAL A ROLE FOR PROTEIN KINASE-C [J].

CONN, PM ;

GANONG, BR ;

EBELING, J ;

STALEY, D ;

NEIDEL, JE ;

BELL, RM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 126 (01) :532-539

[5]

DAVIS RJ, 1985, J BIOL CHEM, V260, P1562

[6] Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities [J].

Dowler, S ;

Currie, RA ;

Campbell, DG ;

Deak, M ;

Kular, G ;

Downes, CP ;

Alessi, DR .

BIOCHEMICAL JOURNAL, 2000, 351 (01) :19-31

[7]

FLORINCHRISTENSEN J, 1992, J BIOL CHEM, V267, P14783

[8] EXIT OF NEWLY SYNTHESIZED MEMBRANE-PROTEINS FROM THE TRANS CISTERNA OF THE GOLGI-COMPLEX TO THE PLASMA-MEMBRANE [J].

GRIFFITHS, G ;

PFEIFFER, S ;

SIMONS, K ;

MATLIN, K .

JOURNAL OF CELL BIOLOGY, 1985, 101 (03) :949-964

[9] Topology and transport of membrane lipids in bacteria [J].

Huijbregts, RPH ;

de Kroon, AIPM ;

de Kruijff, B .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES, 2000, 1469 (01) :43-61

[10] Essential role for diacylglycerol in protein transport from the yeast Golgi complex [J].

Kearns, BG ;

McGee, TP ;

Mayinger, P ;

Gedvilaite, A ;

Phillips, SE ;

Kagiwada, S ;

Bankaitis, VA .

NATURE, 1997, 387 (6628) :101-105

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