Molecular detection of vector-borne pathogens in semen from dogs in southeastern Brazil

被引：0

作者：

Valle, Guilherme Ribeiro ^{[1
]}

Ribeiro, Vitor Marcio ^{[2
]}

Teles, Pedro Paulo de Abreu ^{[3
]}

Ottino, Jennifer ^{[3
]}

Salome, Daniel Magalhaes ^{[2
]}

Melo, Maria Norma ^{[3
]}

Silva, Soraia de Oliveira ^{[3
]}

da Silveira, Julia Angelica Goncalves ^{[3
]}

Dias, Aline Hallais Franca ^{[1
]}

Dantas-Torres, Filipe ^{[4
]}

机构：

[1] Pontificia Univ Catolica Minas Gerais, Dept Med Vet, Rua Santa Rita Durao 1160, BR-30140111 Belo Horizonte, MG, Brazil

[2] Hosp Vet Santo Agostinho, Av Amazonas 2218, BR-30180003 Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil

[4] Fundacao Oswaldo Cruz, Inst Aggeu Magalhaes, Ave Prof Moraes Rego S-N, BR-50670420 Recife, PE, Brazil

来源：

VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS | 2022年 / 36卷

关键词：

Dogs; Males; Sexual transmission; Vectors; Pathogens; TRYPANOSOMA-CRUZI; SEXUAL TRANSMISSION; LEISHMANIA-INFANTUM; DISEASE;

D O I：

10.1016/j.vprsr.2022.100799

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Vector-borne pathogens (VBPs) are primarily transmitted by arthropod vectors, but secondary ways of transmission have been described, including via venereal route. Nonetheless, there is still limited research on possible sexual transmission of VBPs in dogs. We molecularly investigated the presence of vector-borne pathogens in semen from dogs living in an area where these agents are endemic. Upon PCR testing, seven out of 22 (31.8%) semen samples tested positive for at least one VBP, whereas simultaneous positivity to two or more pathogens was detected in three (13.6%) dogs. Among pathogens detected in semen, Trypanosoma cruzi (n = 1) and Leishmania infantum (n = 3) were identified to species level by restriction fragment length polymorphism analysis. Attempts to sequence PCR products from other pathogens were unsuccessful, but coupled epidemiological and molecular data suggest the presence of Anaplasma platys (n = 5), Babesia vogeli (n = 1) and Ehrlichia canis (n = 1) in semen from dogs. Further experimental studies would be needed to confirm the sexual transmission hypothesis for these VBPs and also the possible implications of these findings for canine reproduction.

引用

页数：4

共 65 条

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