Optimization of protein A chromatography for antibody capture

Productivity and buffer consumption of protein A chromatography are important process attributes for antibody capture, driven by industry's need to handle increasing upstream titer and market demand economically. In this work, a model-based optimization approach is presented which evaluates the productivity and buffer consumption of protein A chromatography for antibody capture. The approach offers great potential time and costs savings during process development compared with the traditional experimental approach. To demonstrate the application of the proposed model-based optimization approach, the approach is applied to a case study of antibody capture by a novel industrial silica-based protein A adsorbent.