Nanostructured Superhydrophobic Substrates Trigger the Development of 3D Neuronal Networks

被引：81

作者：

Limongi, Tania ^{[1
,2
]}

Cesca, Fabrizia ^{[2
]}

Gentile, Francesco ^{[1
,4
]}

Marotta, Roberto ^{[3
]}

Ruffilli, Roberta ^{[3
]}

Barberis, Andrea ^{[2
]}

Dal Maschio, Marco ^{[2
]}

Petrini, Enrica Maria ^{[2
]}

Santoriello, Stefania ^{[1
]}

Benfenati, Fabio ^{[2
]}

Di Fabrizio, Enzo ^{[1
]}

机构：

[1] Ist Italiano Tecnol, Dept Nanostruct, I-16163 Genoa, Italy

[2] Ist Italiano Tecnol, Dept Neurosci & Brain Technol, I-16163 Genoa, Italy

[3] Ist Italiano Tecnol, Dept Nanochem, I-16163 Genoa, Italy

[4] Univ Magna Grecia Catanzaro, BioNEM Lab, Dept Expt & Clin Med, I-88100 Catanzaro, Italy

来源：

SMALL | 2013年 / 9卷 / 03期

关键词：

microfabrication; nanostructures; 3D cell cultures; primary neurons; tissue engineering; TOPOGRAPHICAL CONTROL; CELL BEHAVIOR; SURFACES; WETTABILITY; FIBRONECTIN; MOLECULES; ADHESION; GUIDANCE; GROWTH; MICRO;

D O I：

10.1002/smll.201201377

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The generation of 3D networks of primary neurons is a big challenge in neuroscience. Here, a novel method is presented for a 3D neuronal culture on superhydrophobic (SH) substrates. How nano patterned SH devices stimulate neurons to build 3D networks is investigated. Scanning electron microscopy and confocal imaging show that soon after plating neurites adhere to the nanopatterned pillar sidewalls and they are subsequently pulled between pillars in a suspended position. These neurons display an enhanced survival rate compared to standard cultures and develop mature networks with physiological excitability. These findings underline the importance of using nanostructured SH surfaces for directing 3D neuronal growth, as well as for the design of biomaterials for neuronal regeneration.

引用

页码：402 / 412

页数：11

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