Molecular epidemiology and antimicrobial resistance of Clostridioides difficile detected in chicken, soil and human samples from Zimbabwe

被引：14
作者：
Berger, Fabian K. [1 ,2 ]
Mellmann, Alexander [2 ,3 ]
Bischoff, Markus [1 ,2 ]
von Mueller, Lutz [2 ,4 ]
Becker, Soeren L. [1 ]
Simango, Clifford [5 ]
Gaertner, Barbara [1 ,2 ]
机构：
[1] Saarland Univ, Inst Med Microbiol & Hyg, Kirrberger Str,Bldg 43, D-66424 Homburg, Germany
[2] German Natl Reference Ctr Clostridioides Clostrid, Difficile, Germany
[3] Univ Munster, Inst Hyg, Munster, Germany
[4] Christophorus Kliniken, Inst Lab Med Microbiol & Hyg, Coesfeld, Germany
[5] Univ Zimbabwe, Coll Hlth Sci, Dept Med Lab Sci, Harare, Zimbabwe
来源：
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2020年 / 96卷
关键词：
Epidemiology; Surveillance; Zoonosis; cgMLST; Southern Africa; HIGH PREVALENCE; STRAIN; INFECTION; MEAT; DIARRHEA; EMERGENCE; CHILDREN; POULTRY; TCDA;
D O I：
10.1016/j.ijid.2020.04.026
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Background: Clostridioides difficile is the major cause of infectious nosocomial diarrhoea in industrialized nations. Data on the occurrence of C. difficile in Africa, ribotype (RT) distribution, antimicrobial susceptibility patterns and potential zoonotic transmission are scarce. Methods: 80 Zimbabwean C. difficile isolates from different sources (chicken [n=30], soil [n= 21] and humans [n=29]) were investigated using ribotyping, toxin gene detection, resistance testing, multiplelocus variable-number tandem repeat analysis (MLVA), and whole genome sequencing (WGS). Results: Among chicken isolates, the most common RTs were RT103 (6/30), RT025 (5/30) and RT070 (4/30). Within soil samples, RT025 and RT056 were most common (3/21 each). In contrast, the nontoxigenic RT084 was most frequently found in human isolates (4/29). Toxin genes were detected in only 19/29 human isolates. Susceptibility testing showed no resistance against metronidazole and vancomycin, and resistance against macrolides and rifampicin was scarce (3/80 and 2/80, respectively); however, 26/80 isolates showed moxifloxacin resistance. MLVA and WGS of strains with identical RTs stemming from different sources revealed clustering of RT025 and RT084 isolates from human and nonhuman samples. Conclusion: No "hypervirulent" strains were found. The detected clusters between human, chicken and soil isolates indicate ongoing transmission between humans and environmental sources and might point towards a zoonotic potential. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
引用
收藏
页码：82 / 87
页数：6
相关论文
共 53 条
[1]   Presence of Clostridium difficile in poultry and poultry meat in Egypt [J].
Abdel-Glil, Mostafa Y. ;
Thomas, Prasad ;
Schmoock, Gernot ;
Abou-El-Azm, Kamel ;
Wieler, Lothar H. ;
Neubauer, Heinrich ;
Seyboldt, Christian .
ANAEROBE, 2018, 51 :21-25
[2]   The distribution of Clostridium difficile in the environment of South Wales [J].
AlSaif, N ;
Brazier, JS .
JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 45 (02) :133-137
[3]   High prevalence of the epidemic Clostridium difficile PCR ribotype 078 in Iberian free-range pigs [J].
Alvarez-Perez, Sergio ;
Blanco, Jose L. ;
Pelaez, Teresa ;
Astorga, Rafael J. ;
Harmanus, Celine ;
Kuijper, Ed ;
Garcia, Marta E. .
RESEARCH IN VETERINARY SCIENCE, 2013, 95 (02) :358-361
[4]   Prevalence and molecular characterization of Clostridium difficile isolated from European Barn Swallows (Hirundo rustica) during migration [J].
Bandelj, Petra ;
Trilar, Tomi ;
Blagus, Rok ;
Ocepek, Matjaz ;
Rousseau, Joyce ;
Weese, J. Scott ;
Vengust, Modest .
BMC VETERINARY RESEARCH, 2014, 10
[5]   Molecular and culture-based diagnosis of Clostridium difficile isolates from Cote d'Ivoire after prolonged storage at disrupted cold chain conditions [J].
Becker, Soeren L. ;
Chatigre, Justin K. ;
Coulibaly, Jean T. ;
Mertens, Pascal ;
Bonfoh, Bassirou ;
Herrmann, Mathias ;
Kuijper, Ed J. ;
N'Goran, Eliezer K. ;
Utzinger, Juerg ;
von Mueller, Lutz .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2015, 109 (10) :660-668
[6]   Quality assurance for genotyping and resistance testing of Clostridium (Clostridioides) difficile isolates - Experiences from the first inter-laboratory ring trial in four German speaking countries [J].
Berger, Fabian K. ;
Mellmann, Alexander ;
von Mueller, Lutz ;
Bischoff, Markus ;
Gaertner, Barbara C. .
ANAEROBE, 2020, 61
[7]   Molecular characterization, toxin detection and resistance testing of human clinical Clostridium difficile isolates from Lebanon [J].
Berger, Fabian K. ;
Rasheed, Sari S. ;
Araj, George F. ;
Mahfouz, Rami ;
Rimmani, Hussein H. ;
Karaoui, Walid R. ;
Sharara, Ala I. ;
Dbaibo, Ghassan ;
Becker, Soeren L. ;
von Mueller, Lutz ;
Bischoff, Markus ;
Matar, Ghassan M. ;
Gartner, Barbara .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2018, 308 (03) :358-363
[8]  
Berger FK, 2019, INT J MED MICROBIOL
[9]  
Bletz S, 2018, J CLIN MICROBIOL, V56, DOI [10.1128/JCM.01987-17, 10.1128/jcm.01987-17]
[10]   Comparative Genome Analysis and Global Phylogeny of the Toxin Variant Clostridium difficile PCR Ribotype 017 Reveals the Evolution of Two Independent Sublineages [J].
Cairns, M. D. ;
Preston, M. D. ;
Hall, C. L. ;
Gerding, D. N. ;
Hawkey, P. M. ;
Kato, H. ;
Kim, H. ;
Kuijper, E. J. ;
Lawley, T. D. ;
Pituch, H. ;
Reid, S. ;
Kullin, B. ;
Riley, T. V. ;
Solomon, K. ;
Tsai, P. J. ;
Weese, J. S. ;
Stabler, R. A. ;
Wren, B. W. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (03) :865-876
123456