Relative control of soil moisture [30, 60, and 80 percent water-holding capacity (WHC)] on nitrous oxide (N2O) emissions from Fargo-Ryan soil, treated with urea at 0, 150, and 250kgNha(-1) with and without nitrapyrin [2-chloro-(6-trichloromethyl) pyridine] (NP), was measured under laboratory condition for 140days. Soil N2O emissions significantly increased with increasing nitrogen (N) rates and WHC levels. Urea applied at 250kgNha(-1) produced the greatest cumulative N2O emissions and averaged 560, 3919, and 15894 mu gkg(-1) at 30, 60, and 80 percent WHC, respectively. At WHC <= 60 percent, addition of NP to urea significantly reduced N2O losses by 2.6- to 4.8-fold. Additions of NP to urea reduced N2O emission at rates similar to the control (0N) until 48days for 30 percent WHC and 35days for 60 and 80 percent WHC. These results can help devise urea-N fertilizer management strategies in reducing N2O emissions from silty-clay soils.