Antibiofilm Activity of Weissella spp. and Bacillus coagulans Isolated from Equine Skin against Staphylococcus aureus

被引：6

作者：

Stykova, Eva ^{[1
]}

Nemcova, Radomira ^{[2
]}

Maar, Marian ^{[2
]}

Bujnakova, Dobroslava ^{[3
]}

Mucha, Rastislav ^{[4
]}

Gancarcikova, Sona ^{[2
]}

Requena Domenech, Francisco ^{[5
]}

机构：

[1] Univ Vet Med & Pharm Kosice, Univ Vet Hosp, Clin Horses, Komenskeho 73, Kosice 04181, Slovakia

[2] Univ Vet Med & Pharm Kosice, Dept Microbiol & Immunol, Komenskeho 73, Kosice 04181, Slovakia

[3] Slovak Acad Sci, Inst Anim Physiol, Ctr Biosci, Soltesovej 4, Kosice 04001, Slovakia

[4] Slovak Acad Sci, Inst Neurobiol, Biomed Res Ctr, Soltesovej 4, Kosice 04001, Slovakia

[5] Univ Cordoba, Dept Cell Biol Physiol & Immunol, Cordoba 14014, Spain

来源：

LIFE-BASEL | 2022年 / 12卷 / 12期

关键词：

antibiofilm activity; antimicrobial activity; Bacillus coagulans; probiotic; skin; Weissella spp; NEWLY DISCOVERED BACTERIOCIN; LACTIC-ACID BACTERIA; BIOFILM FORMATION; ACETIC-ACID; STRAINS; BIOSURFACTANTS; MECHANISMS; LACTOBACILLUS; LEUCONOSTOC; METABOLITES;

D O I：

10.3390/life12122135

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to evaluate the antimicrobial and antibiofilm activity of Weissella cibaria, Weissella hellenica and Bacillus coagulans, isolated from equine skin, against biofilm-forming Staphylococcus aureus CCM 4223 and clinical isolate methicillin-resistant S. aureus (MRSA). Non-neutralized cell-free supernatants (nnCFS) of tested skin isolates completely inhibited the growth and biofilm formation of S. aureus strains and caused dispersion of the 24 h preformed biofilm in the range of 21-90%. The majority of the pH-neutralized cell-free supernatants (nCFS) of skin isolates inhibited the biofilm formation of both S. aureus strains in the range of 20-100%. The dispersion activity of B. coagulans nCFS ranged from 17 to 77% and was significantly lower than that of nnCFS, except for B. coagulans 3T27 against S. aureus CCM 4223. Changes in the growth of S. aureus CCM 4223 in the presence of catalase- or trypsin-treated W. hellenica 4/2D23 and W. cibaria 4/8D37 nCFS indicated the role of peroxides and/or bacteriocin in their antimicrobial activities. For the first time, the presence of the fenD gene, associated with biosurfactants production, was detected in B. coagulans. The results of this study showed that selected isolates may have the potential for the prevention and treatment of biofilm-forming S. aureus infections.

引用

页数：20

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