Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases

被引：127

作者：

Berchtold, S

Ogilvie, ALJ

Bogdan, C

Mühl-Zürbes, P

Ogilvie, A

Schuler, G

Steinkasserer, A

机构：

[1] Univ Erlangen Nurnberg, Dept Dermatol, D-91052 Erlangen, Germany

[2] Univ Erlangen Nurnberg, Dept Biochem, D-8520 Erlangen, Germany

来源：

FEBS LETTERS | 1999年 / 458卷 / 03期

关键词：

dendritic cell; ATP; P2; receptor; ecto-nucleotidase;

D O I：

10.1016/S0014-5793(99)01197-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the expression and function of P2 receptors and ecto-nucleotidases on human monocyte derived dendritic cells (DC), In addition we analyzed the effect of extracellular ATP on the maturation of DC. By RT-PCR, DC Here found to express mRNA for several P2X (P2X1, P2X4, P2X5, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y5, P2Y6, P2Y10, P2Y11) receptors, AS shown by FURA-2 measurement, triggering of P2 receptors resulted in an increase in free intracellular Ca2+. In combination with Tumor necrosis factor-alpha ATP increased the expression of the DC surface markers CD80, CD83 and CD86 indicating a maturation promoting effect. DC expressed the ecto-apyrase CD39 and the ecto-5'-nucleotidase CD73 as demonstrated by RT-PCR, Extracellular ATP was rapidly hydrolyzed by these ecto-enzymes as shown by separation of H-3-labeled ATP metabolites using a thin layer technique. These data suggest that ATP acts as a costimulatory factor on DC maturation. (C) 1999 Federation of European Biochemical Societies.

引用

收藏

页码：424 / 428

页数：5

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