A Mutation in the Stalk of the Newcastle Disease Virus Hemagglutinin-Neuraminidase (HN) Protein Prevents Triggering of the F Protein despite Allowing Efficient HN-F Complex Formation

被引：23

作者：

Mirza, Anne M. ^{[1
]}

Iorio, Ronald M. ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Microbiol & Physiol Syst, Immunol & Microbiol Program, Worcester, MA 01605 USA

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 15期

基金：

美国国家卫生研究院;

关键词：

HOMOLOGOUS FUSION PROTEIN; MONOCLONAL-ANTIBODIES; PARAMYXOVIRUS FUSION; RECEPTOR RECOGNITION; SENDAI-VIRUS; CELL-FUSION; GLYCOPROTEIN; DOMAIN; PROMOTION; FORM;

D O I：

10.1128/JVI.01066-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Newcastle disease virus (NDV)-induced membrane fusion requires formation of a complex between the hemagglutinin-neuraminidase (HN) and fusion (F) proteins. Substitutions for NDV HN stalk residues A89, L90, and L94 block fusion by modulating formation of the HN-F complex. Here, we demonstrate that a nearby L97A substitution, though previously shown to block fusion, allows efficient HN-F complex formation and likely acts by preventing changes in the HN stalk required for triggering of the bound F protein.

引用

页码：8813 / 8815

页数：3

共 25 条

[1] Fusion activation by a headless parainfluenza virus 5 hemagglutinin-neuraminidase stalk suggests a modular mechanism for triggering [J].

Bose, Sayantan ;

Zokarkar, Aarohi ;

Welch, Brett D. ;

Leser, George P. ;

Jardetzky, Theodore S. ;

Lamb, Robert A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (39) :E2625-E2634

[2] Triggering the measles virus membrane fusion machinery [J].

Brindley, Melinda A. ;

Takeda, Makoto ;

Plattet, Philippe ;

Plemper, Richard K. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (44) :E3018-E3027

[3] Mutations in the stalk of the measles virus hemagglutinin protein decrease fusion but do not interfere with virus-specific interaction with the homologous fusion protein [J].

Corey, Elizabeth A. ;

Iorio, Ronald M. .

JOURNAL OF VIROLOGY, 2007, 81 (18) :9900-9910

[4]

Deng R, 1997, ARCH VIROL, P115

[5] LOCALIZATION OF A DOMAIN ON THE PARAMYXOVIRUS ATTACHMENT PROTEIN REQUIRED FOR THE PROMOTION OF CELLULAR FUSION BY ITS HOMOLOGOUS FUSION PROTEIN SPIKE [J].

DENG, RT ;

WANG, ZY ;

MIRZA, AM ;

IORIO, RM .

VIROLOGY, 1995, 209 (02) :457-469

[6] EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].

FUERST, TR ;

NILES, EG ;

STUDIER, FW ;

MOSS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126

[7] FUNCTIONAL AND NEUTRALIZATION PROFILE OF 7 OVERLAPPING ANTIGENIC SITES ON THE HN GLYCOPROTEIN OF NEWCASTLE-DISEASE VIRUS - MONOCLONAL-ANTIBODIES TO SOME SITES PREVENT VIRAL ATTACHMENT [J].

IORIO, RM ;

GLICKMAN, RL ;

RIEL, AM ;

SHEEHAN, JP ;

BRATT, MA .

VIRUS RESEARCH, 1989, 13 (03) :245-261

[8] NEUTRALIZATION MAP OF THE HEMAGGLUTININ-NEURAMINIDASE GLYCOPROTEIN OF NEWCASTLE-DISEASE VIRUS - DOMAINS RECOGNIZED BY MONOCLONAL-ANTIBODIES THAT PREVENT RECEPTOR RECOGNITION [J].

IORIO, RM ;

SYDDALL, RJ ;

SHEEHAN, JP ;

BRATT, MA ;

GLICKMAN, RL ;

RIEL, AM .

JOURNAL OF VIROLOGY, 1991, 65 (09) :4999-5006

[9] GENETIC-VARIATION WITHIN A NEUTRALIZING DOMAIN ON THE HEMAGGLUTININ NEURAMINIDASE GLYCOPROTEIN OF NEWCASTLE-DISEASE VIRUS [J].

IORIO, RM ;

BORGMAN, JB ;

GLICKMAN, RL ;

BRATT, MA .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1393-1403

[10] MONOCLONAL-ANTIBODIES TO NEWCASTLE-DISEASE VIRUS - DELINEATION OF 4 EPITOPES ON THE HN GLYCOPROTEIN [J].

IORIO, RM ;

BRATT, MA .

JOURNAL OF VIROLOGY, 1983, 48 (02) :440-450

← 1 2 3 →