Phospholipase A2 catalysis and lipid mediator lipidomics

被引：99

作者：

Mouchlis, Varnavas D. ^{[1
]}

Dennis, Edward A. ^{[1
]}

机构：

[1] Univ Calif San Diego, Sch Med, Dept Pharmacol, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2019年 / 1864卷 / 06期

关键词：

Phospholipase; Catalytic mechanism; HD-XMS; Molecular dynamics; LC/MS assays; Substrate specificity; EXCHANGE MASS-SPECTROMETRY; PLATELET-ACTIVATING-FACTOR; GROUP IVA; HYDROGEN/DEUTERIUM EXCHANGE; GROUP-V; ARACHIDONIC-ACID; PROSTAGLANDIN PRODUCTION; MEMBRANE ORGANIZATION; MOLECULAR DOCKING; NUCLEAR-ENVELOPE;

D O I：

10.1016/j.bbalip.2018.08.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipase A(2) (PLA(2)) enzymes are the upstream regulators of the eicosanoid pathway liberating free arachidonic acid from the sn-2 position of membrane phospholipids. Free intracellular arachidonic acid serves as a substrate for the eicosanoid biosynthetic enzymes including cyclooxygenases, lipoxygenases, and cytochrome P450s that lead to inflammation. The Group IVA cytosolic (cPLA(2)), Group VIA calcium-independent (iPLA(2)), and Group V secreted (sPLA(2)) are three well-characterized human enzymes that have been implicated in eicosanoid formation. In this review, we will introduce and summarize the regulation of catalytic activity and cellular localization, structural characteristics, interfacial activation and kinetics, substrate specificity, inhibitor binding and interactions, and the downstream implications for eicosanoid biosynthesis of these three important PLA(2) enzymes.

引用

页码：766 / 771

页数：6

共 67 条

[1]

ACKERMANN EJ, 1994, J BIOL CHEM, V269, P9227

[2] Localization of Group V phospholipase A2 in caveolin-enriched granules in activated P388D1 macrophage-like cells [J].

Balboa, MA ;

Shirai, Y ;

Gaietta, G ;

Ellisman, MH ;

Balsinde, J ;

Dennis, EA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (48) :48059-48065

[3] Antisense inhibition of group VI Ca2+-independent phospholipase A(2) blocks phospholipid fatty acid remodeling in murine P388D(1) macrophages [J].

Balsinde, J ;

Balboa, MA ;

Dennis, EA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (46) :29317-29321

[4] Arachidonic acid is preferentially metabolized by cyclooxygenase-2 to prostacyclin and prostaglandin E2 [J].

Brock, TG ;

McNish, RW ;

Peters-Golden, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (17) :11660-11666

[5] Insertion of the Ca2+-Independent Phospholipase A2 into a Phospholipid Bilayer via Coarse-Grained and Atomistic Molecular Dynamics Simulations [J].

Bucher, Denis ;

Hsu, Yuan-Hao ;

Mouchlis, Varnavas D. ;

Dennis, Edward A. ;

McCammon, J. Andrew .

PLOS COMPUTATIONAL BIOLOGY, 2013, 9 (07)

[6] An integrated omics analysis of eicosanoid biology [J].

Buczynski, Matthew W. ;

Dumlao, Darren S. ;

Dennis, Edward A. .

JOURNAL OF LIPID RESEARCH, 2009, 50 (06) :1015-1038

[7] A Phospholipid Substrate Molecule Residing in the Membrane Surface Mediates Opening of the Lid Region in Group IVA Cytosolic Phospholipase A2 [J].

Burke, John E. ;

Hsu, Yuan-Hao ;

Deems, Raymond A. ;

Li, Sheng ;

Woods, Virgil L., Jr. ;

Dennis, Edward A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (45) :31227-31236

[8] Interaction of group IA phospholipase A2 with metal ions and phospholipid vesicles probed with deuterium exchange mass spectrometry [J].

Burke, John E. ;

Karbarz, Mark J. ;

Deems, Raymond A. ;

Li, Sheng ;

Woods, Virgil L. ;

Dennis, Edward A. .

BIOCHEMISTRY, 2008, 47 (24) :6451-6459

[9] Location of Inhibitors Bound to Group IVA Phospholipase A2 Determined by Molecular Dynamics and Deuterium Exchange Mass Spectrometry [J].

Burke, John E. ;

Babakhani, Arneh ;

Gorfe, Alemayehu A. ;

Kokotos, George ;

Li, Sheng ;

Woods, Virgil L., Jr. ;

McCammon, J. Andrew ;

Dennis, Edward A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (23) :8083-8091

[10] Using Hydrogen/Deuterium Exchange Mass Spectrometry to Define the Specific Interactions of the Phospholipase A2 Superfamily with Lipid Substrates, Inhibitors, and Membranes [J].

Cao, Jian ;

Burke, John E. ;

Dennis, Edward A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (03) :1806-1813

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