Ehrlichia canis in dogs in a semiarid region of Northeastern Brazil: Serology, molecular detection and associated factors

被引：23

作者：

Tanikawa, A. ^{[1
]}

Labruna, M. B. ^{[2
]}

Costa, A. ^{[2
]}

Aguiar, D. M. ^{[3
]}

Justiniano, S. V. ^{[1
]}

Mendes, R. S. ^{[1
,2
,3
]}

Melo, A. L. T. ^{[3
]}

Alves, C. J. ^{[1
]}

Azevedo, S. S. ^{[1
]}

机构：

[1] Univ Fed Campina Grande, Ctr Saude & Tecnol Rural, Unidade Acad Med Vet, Patos de Minas, PB, Brazil

[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, Brazil

[3] Univ Fed Mato Grosso, Fac Agron Med Vet & Zootecnia, Dept Clin Med Vet, Cuiaba, MT, Brazil

来源：

RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 03期

关键词：

Ehrlichia canis; Serology; PCR; Dogs; Northeastern Brazil; TICKS; PREVALENCE;

D O I：

10.1016/j.rvsc.2012.10.007

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

This study investigated infection by Ehrlichia spp. agents by PCR, immunofluorescence assay test (IFAT), and by Giemsa-stained blood smears in 108 dogs from a semiarid region of the state of Paraiba, North-eastern Brazil. Seventy-five (69.4%) of the 108 dogs were found to be seropositive to Ehrlichia canis, while only four dogs (3.7%) were positive in real-time PCR for E. canis. In six dogs (5.6%) E. canis-like morulae were observed in monocytes. Animals that stayed in environment whose floor was dried dirt, and dogs whose owners reported low frequency of cleaning the dog environment had higher (P < 0.05) PCR positivity for E. canis. Increasing seropositivity was found in older dogs (P = 0.012). This study provides the first molecular detection of E. canis in the semiarid region of Northeastern Brazil. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：474 / 477

页数：4

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