Rapid and sensitive detection of bacteria via platinum-labeled antibodies and on-particle ionization and enrichment prior to MALDI-TOF mass spectrometry

被引：3

作者：

Ahmad, Faheem ^{[1
]}

Wu, Hui-Fen ^{[1
,2
,3
,4
]}

机构：

[1] Natl Sun Yat Sen Univ, Dept Chem, Kaohsiung 80424, Taiwan

[2] Natl Sun Yat Sen Univ, Ctr Nanosci & Nanotechnol, Kaohsiung 80424, Taiwan

[3] Natl Sun Yat Sen Univ, Doctoral Degree Program Marine Biotechnol, Kaohsiung 80424, Taiwan

[4] Kaohsiung Med Univ, Sch Pharm, Coll Pharm, Kaohsiung 807, Taiwan

来源：

MICROCHIMICA ACTA | 2013年 / 180卷 / 5-6期

关键词：

IgG-Pt NPs; MALDI-MS; S; marcescens; E; coli; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; PROTEIN-A; NANOPARTICLES; ENTEROBACTERIACEAE; IDENTIFICATION;

D O I：

10.1007/s00604-013-0951-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We introduce a rapid and sensitive approach to study the interactions of an affinity probe with the bacterial wall. Immunoglobulin was immobilized on platinum nanoparticles, and the resulting probe nanoparticles bind to bacterial walls as confirmed by transmission electron microscopy. A MALDI-MS assay was developed that can detect similar to 10(5) cfu mL(-1) of S. marcescens and E. coli. This approach enables simple, rapid and straightforward detection of bacterial proteins, with high resolution and sensitivity, and without the requirement for tedious washing/separation steps.

引用

页码：485 / 492

页数：8

共 27 条

[1] Rapid and highly sensitive detection of single nematode via direct MALDI Mass Spectrometry [J].

Ahmad, Faheem ;

Gopal, Judy ;

Wu, Hui-Fen .

TALANTA, 2012, 93 :182-185

[2] High-resolution MALDI-TOF mass spectrometry of bacterial proteins using a Tris-EDTA buffer approach [J].

Ahmad, Faheem ;

Wu, Hui-Fen .

MICROCHIMICA ACTA, 2012, 176 (3-4) :311-316

[3] Characterization of pathogenic bacteria using ionic liquid via single drop microextraction combined with MALDI-TOF MS [J].

Ahmad, Faheem ;

Wu, Hui-Fen .

ANALYST, 2011, 136 (19) :4020-4027

[4] Electrostatic layer-by-layer nanoassembly on biological microtemplates: Platelets [J].

Ai, H ;

Fang, M ;

Jones, SA ;

Lvov, YM .

BIOMACROMOLECULES, 2002, 3 (03) :560-564

[5]

Arnold R, 1998, P 46 ASMS C MASS SPE, P180

[6] Species Differentiation of Seafood Spoilage and Pathogenic Gram-Negative Bacteria by MALDI-TOF Mass Fingerprinting [J].

Boehme, Karola ;

Fernandez-No, Inmaculada C. ;

Barros-Velazquez, Jorge ;

Gallardo, Jose M. ;

Calo-Mata, Pilar ;

Canas, Benito .

JOURNAL OF PROTEOME RESEARCH, 2010, 9 (06) :3169-3183

[7] Detection of pathogenic Staphylococcus aureus bacteria by gold based immunosensors [J].

Boujday, Souhir ;

Briandet, Romain ;

Salmain, Michele ;

Herry, Jean-Marie ;

Marnet, Pierre-Guy ;

Gautier, Michel ;

Pradier, Claire-Marie .

MICROCHIMICA ACTA, 2008, 163 (3-4) :203-209

[8] Synthesis of thiolate-stabilized platinum nanoparticles in protolytic solvents as isolable colloids [J].

Chen, SH ;

Kimura, K .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (23) :5397-5403

[9]

[Department of Health and Human Services Centers for Disease Control and Prevention National Institute for Occupational Safety and Health], 2009, APPR SAF NON

[10] Surface plasmon resonance: principles, methods and applications in biomedical sciences [J].

Englebienne, P ;

Van Hoonacker, A ;

Verhas, M .

SPECTROSCOPY-AN INTERNATIONAL JOURNAL, 2003, 17 (2-3) :255-273

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