Tubulin Tyrosine Ligase Like 12, a TTLL Family Member with SET- and TTL-Like Domains and Roles in Histone and Tubulin Modifications and Mitosis

被引：22

作者：

Brants, Jan ^{[1
]}

Semenchenko, Kostyantyn ^{[1
]}

Wasylyk, Christine ^{[1
]}

Robert, Aude ^{[2
]}

Carles, Annaick ^{[1
]}

Zambrano, Alberto ^{[1
]}

Pradeau-Aubreton, Karine ^{[1
]}

Birck, Catherine ^{[1
]}

Schalken, Jack A. ^{[3
]}

Poch, Olivier ^{[1
]}

de Mey, Jan ^{[2
]}

Wasylyk, Bohdan ^{[1
]}

机构：

[1] Inst Genet & Biol Mol & Cellulaire, UMR CNRS UDS INSERM 7104 U964, Illkirch Graffenstaden, France

[2] Univ Strasbourg, Ecole Super Biotechnol Strasbourg CNRS UMR7100, Equipe Microtubules & Morphogenese, Illkirch Graffenstaden, France

[3] Radboud Univ Nijmegen, Med Ctr, Dept Urol, NL-6525 ED Nijmegen, Netherlands

来源：

PLOS ONE | 2012年 / 7卷 / 12期

关键词：

PROTEIN LYSINE METHYLTRANSFERASES; CELL-CYCLE; CHROMOSOME SEGREGATION; MAMMALIAN-CELLS; POSTTRANSLATIONAL MODIFICATIONS; CONSTITUTIVE HETEROCHROMATIN; MICROTUBULE CYTOSKELETON; METHYLATION; PR-SET7; ENZYMES;

D O I：

10.1371/journal.pone.0051258

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

hTTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. It has both SET-like and TTL-like domains, suggesting that it could have histone methylation and tubulin tyrosine ligase activities. Altered expression of hTTLL12 in human cells leads to specific changes in H4K20 trimethylation, and tubulin detyrosination, hTTLL12 does not catalyse histone methylation or tubulin tyrosination in vitro, as might be expected from the lack of critical amino acids in its SET-like and TTLL-like domains. hTTLL12 misexpression increases mitotic duration and chromosome numbers. These results suggest that hTTLL12 has non-catalytic functions related to tubulin and histone modification, which could be linked to its effects on mitosis and chromosome number stability.

引用

页数：13

共 59 条

[1] New lives for old: evolution of pseudoenzyme function illustrated by iRhoms [J].

Adrain, Colin ;

Freeman, Matthew .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2012, 13 (08) :489-498

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3] Natural History of the Eukaryotic Chromatin Protein Methylation System [J].

Aravind, L. ;

Abhiman, Saraswathi ;

Iyer, Lakshminarayan M. .

MODIFICATIONS OF NUCLEAR DNA AND ITS REGULATORY PROTEINS, 2011, 101 :105-176

[4] Biotin carboxylase comes into the fold [J].

Artymiuk, PJ ;

Poirrette, AR ;

Rice, DW ;

Willett, P .

NATURE STRUCTURAL BIOLOGY, 1996, 3 (02) :128-132

[5] PR-Set7 and H4K20me1: at the crossroads of genome integrity, cell cycle, chromosome condensation, and transcription [J].

Beck, David B. ;

Oda, Hisanobu ;

Shen, Steven S. ;

Reinberg, Danny .

GENES & DEVELOPMENT, 2012, 26 (04) :325-337

[6] Insights into fetal and neonatal development through analysis of cell-free RNA in body fluids [J].

Bianchi, Diana W. ;

Maron, Jill L. ;

Johnson, Kirby L. .

EARLY HUMAN DEVELOPMENT, 2010, 86 (11) :747-752

[7] Coupling mitosis to DNA replication: The emerging role of the histone H4-lysine 20 methyltransferase PR-Set7 [J].

Brustel, Julien ;

Tardat, Mathieu ;

Kirsh, Olivier ;

Grimaud, Charlotte ;

Julien, Eric .

TRENDS IN CELL BIOLOGY, 2011, 21 (08) :452-460

[8] Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display [J].

Carles, A ;

Millon, R ;

Cromer, A ;

Ganguli, G ;

Lemaire, F ;

Young, J ;

Wasylyk, C ;

Muller, D ;

Schultz, I ;

Rabouel, Y ;

Dembélé, D ;

Zhao, C ;

Marchal, P ;

Ducray, C ;

Bracco, L ;

Abecassis, J ;

Poch, O ;

Wasylyk, B .

ONCOGENE, 2006, 25 (12) :1821-1831

[9] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[10] Structural dynamics of protein lysine methylation and demethylation [J].

Cheng, Xiaodong ;

Zhang, Xing .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2007, 618 (1-2) :102-115

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