A helix scaffold for the assembly of active protein kinases

被引:344
作者
Kornev, Alexandr P. [1 ]
Taylor, Susan S. [1 ,2 ]
Ten Eyck, Lynn F. [2 ,3 ]
机构
[1] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, San Diego Supercomp Ctr, La Jolla, CA 92093 USA
基金
美国国家卫生研究院;
关键词
graph theory; hydrophobic motifs; structure comparison;
D O I
10.1073/pnas.0807988105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Structures of set of serine-threonine and tyrosine kinases were investigated by the recently developed bioinformatics tool Local Spatial Patterns (LSP) alignment. We report a set of conserved motifs comprised mostly of hydrophobic residues. These residues are scattered throughout the protein sequence and thus were not previously detected by traditional methods. These motifs traverse the conserved protein kinase core and play integrating and regulatory roles. They are anchored to the F-helix, which acts as an organizing "hub" providing precise positioning of the key catalytic and regulatory elements. Consideration of these discovered structures helps to explain previously inexplicable results.
引用
收藏
页码:14377 / 14382
页数:6
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