C/EBP homologous protein (CHOP) mediates neuronal apoptosis in rats with spinal cord injury

被引：29

作者：

Wang, Zhangfu ^{[1
]}

Zhang, Chuanyi ^{[1
]}

Hong, Zhenghua ^{[1
]}

Chen, Haixiao ^{[1
]}

Chen, Weifu ^{[1
]}

Chen, Guofu ^{[1
]}

机构：

[1] Taizhou Hosp Zhejiang Prov, Dept Orthoped, Taizhou 317000, Zhejiang, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2013年 / 5卷 / 01期

关键词：

C/EBP homologous protein; neuron; spinal cord injury; apoptosis; ENDOPLASMIC-RETICULUM STRESS; CELL-DEATH; NERVOUS-SYSTEM; REDUCES APOPTOSIS; ISCHEMIA; DEGENERATION; CHOP/GADD153; INVOLVEMENT; INHIBITION; PATHWAYS;

D O I：

10.3892/etm.2012.745

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Spinal cord injury (SCI) is a severe health problem and the mechanism involved remains elusive. The aim of the present study was to elucidate the role of C/EBP homologous protein (CHOP), a prominent protein of the endoplasmic reticulum (ER) stress-mediated apoptosis in SCI. A total of 20 adult male Sprague-Dawley rats were divided into two groups at random, ten rats were subjected to a modified Allen's test (using a weight-drop device) to induce a SCI model and the remaining ten rats only had the corresponding vertebral lamina removed with no injury and served as the sham-operated group. Pathological changes in the spinal cord were observed 12 h after injury by hematoxylin and eosin staining and TUNEL staining was performed to visualize apoptotic cells. The expression of CHOP was also detected by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction. The results showed that a typical apoptotic morphology, namely the increased the number of TUNEL-positive cells in the injured spinal cord. The expression levels of CHOP in the rats with SCI were increased compared with the sham-operated rats (P<0.05). These results revealed that CHOP-mediated ER stress-induced apoptosis may be involved in SCI.

引用

页码：107 / 111

页数：5

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