Manipulation of gene expression in zebrafish using caged circular morpholino oligomers

被引：50

作者：

Wang, Yuan ^{[1
]}

Wu, Li ^{[1
]}

Wang, Peng ^{[1
]}

Lv, Cong ^{[1
]}

Yang, Zhenjun ^{[1
]}

Tang, Xinjing ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

来源：

NUCLEIC ACIDS RESEARCH | 2012年 / 40卷 / 21期

基金：

美国国家科学基金会;

关键词：

PHOTOCHEMICAL CONTROL; RNA DIGESTION; IN-VITRO; LIGHT; ACTIVATION; PHOTOREGULATION; AZOBENZENE; TRANSCRIPTION; DEACTIVATION; POLYMERASE;

D O I：

10.1093/nar/gks840

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate beta-catenin-2 and no tail expression in zebrafish embryos.

引用

页码：11155 / 11162

页数：8

共 58 条

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Ando H, 2004, METHOD CELL BIOL, V77, P159

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