Characterization of an Entner-Doudoroff pathway-activated Escherichia coli

被引:7
作者
Kim, Ye Eun [1 ]
Cho, Kyung Hyun [1 ]
Bang, Ina [1 ]
Kim, Chang Hee [2 ]
Ryu, Young Shin [1 ]
Kim, Yuchan [1 ]
Choi, Eun Mi [1 ]
Nong, Linh Khanh [1 ]
Kim, Donghyuk [1 ,2 ]
Lee, Sung Kuk [1 ,2 ]
机构
[1] Ulsan Natl Inst Sci & Technol UNIST, Sch Energy & Chem Engn, Ulsan 44919, South Korea
[2] UNIST, Dept Biomed Engn, Ulsan 44919, South Korea
来源
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS | 2022年 / 15卷 / 01期
基金
新加坡国家研究基金会;
关键词
Escherichia coli; Glucose metabolism; Entner-Doudoroff pathway; Adaptive laboratory evolution; Phosphofructokinase; 3-Hydroxypropionic acid; PENTOSE-PHOSPHATE PATHWAY; REDUCTIVE BIOTRANSFORMATION; PHOSPHOGLUCOSE ISOMERASE; 3-HYDROXYPROPIONIC ACID; CARBOHYDRATE-METABOLISM; GLUCOSE-TRANSPORT; ZYMOMONAS-MOBILIS; SMALL RNA; EXPRESSION; GENE;
D O I
10.1186/s13068-022-02219-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background Escherichia coli have both the Embden-Meyerhof-Parnas pathway (EMPP) and Entner-Doudoroff pathway (EDP) for glucose breakdown, while the EDP primarily remains inactive for glucose metabolism. However, EDP is a more favorable route than EMPP for the production of certain products. Results EDP was activated by deleting the pfkAB genes in conjunction with subsequent adaptive laboratory evolution (ALE). The evolved strains acquired mutations in transcriptional regulatory genes for glycolytic process (crp, galR, and gntR) and in glycolysis-related genes (gnd, ptsG, and talB). The genotypic, transcriptomic and phenotypic analyses of those mutations deepen our understanding of their beneficial effects on cellulosic biomass bio-conversion. On top of these scientific understandings, we further engineered the strain to produce higher level of lycopene and 3-hydroxypropionic acid. Conclusions These results indicate that the E. coli strain has innate capability to use EDP in lieu of EMPP for glucose metabolism, and this versatility can be harnessed to further engineer E. coli for specific biotechnological applications.
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