Lipoic acid prevents steroid-induced osteonecrosis in rabbits

被引:25
作者
Lu, Bang-Bao [1 ]
Li, Kang-Hua [1 ]
机构
[1] Cent S Univ, Dept Orthoped, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China
关键词
Osteonecrosis; Oxidative stress; Prevention; Rabbit; Steroid; APOPTOTIC CELL-DEATH; OXIDATIVE STRESS; FEMORAL-HEAD; BONE; PATHOGENESIS; NECROSIS;
D O I
10.1007/s00296-011-1846-6
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The objective of this study was to investigate in vivo effects of lipoic acid (LA) in preventing steroid-induced osteonecrosis and the possible pathway in a rabbit model. Sixty rabbits were divided into 2 groups: rabbits were intraperitoneally injected with LA aqueous solution at 36 mg/kg of body weight per day for 4 weeks in Group A and rabbits were injected with physiologic saline (PS) as a control in Group B. At 2 weeks after starting treatment, they were intramuscularly injected once with 20 mg/kg of methylprednisolone acetate (MPSL). The femora were histopathologically examined for the presence of osteonecrosis. The plasma levels of total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), glutathione (GSH), endothelin (ET) and malondialdehyde (MDA) were assayed at 2 weeks after the injection of MPSL. The incidence of osteonecrosis was significantly higher in Group B (73.1%) than in Group A (20.8%). The GSH level was higher in Group A than in Group B after the LA injection. The plasma MDA and ET levels were lower in Group A than in Group B at 2 weeks after the MPSL administration. Lipoic acid can prevent the development of steroid-induced osteonecrosis in rabbits. Inhibited oxidative stress and amendment of vascular endothelial dysfunction is a possible mechanism for this effect.
引用
收藏
页码:1679 / 1683
页数:5
相关论文
共 23 条
[1]   ASEPTIC NECROSIS OF BONE IN SYSTEMIC LUPUS-ERYTHEMATOSUS - RELATIONSHIP TO CORTICOSTEROID-THERAPY [J].
ABELES, M ;
URMAN, JD ;
ROTHFIELD, NF .
ARCHIVES OF INTERNAL MEDICINE, 1978, 138 (05) :750-754
[2]   ROLE OF OXIDATIVE STRESS IN DEVELOPMENT OF COMPLICATIONS IN DIABETES [J].
BAYNES, JW .
DIABETES, 1991, 40 (04) :405-412
[3]   Ex vivo induction of apoptosis in lymphocytes is mediated by oxidative stress: Role for lymphocyte loss in HIV infection [J].
Dobmeyer, TS ;
Findhammer, S ;
Dobmeyer, JM ;
Klein, SA ;
Raffel, B ;
Hoelzer, D ;
Helm, EB ;
Kabelitz, D ;
Rossol, R .
FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :775-785
[4]   Redox regulation of apoptotic cell death [J].
Hampton, MB ;
Orrenius, S .
BIOFACTORS, 1998, 8 (1-2) :1-5
[5]   DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis [J].
Ichiseki, T ;
Kaneuji, A ;
Katsuda, S ;
Ueda, Y ;
Sugimori, T ;
Matsumoto, T .
RHEUMATOLOGY, 2005, 44 (04) :456-460
[6]   Osteonecrosis induced by a single administration of low-dose lipopolysaccharide in rabbits [J].
Irisa, T ;
Yamamoto, T ;
Miyanishi, K ;
Yamashita, A ;
Iwamoto, Y ;
Sugioka, Y ;
Sueishi, K .
BONE, 2001, 28 (06) :641-649
[7]   Effect of simvastatin on steroid-induced osteonecrosis evidenced by the serum lipid level and hepatic cytochrome P4503A in a rabbit model [J].
Iwakiri, Kentaro ;
Oda, Yutaka ;
Kaneshiro, Yasunori ;
Iwaki, Hiroyoshi ;
Masada, Toshiaki ;
Kobayashi, Akio ;
Asada, Akira ;
Takaoka, Kunio .
JOURNAL OF ORTHOPAEDIC SCIENCE, 2008, 13 (05) :463-468
[8]  
JONES JP, 1994, AAOS INSTR COURS LEC, V43, P499
[9]  
JONES JP, 1993, CLIN ORTHOP RELAT R, P294
[10]  
Kabata T, 2000, J RHEUMATOL, V27, P2166