The Cysteine-rich Region of Type VII Collagen Is a Cystine Knot with a New Topology*

Background: Type VII collagen is essential for skin stability as highlighted by related skin blistering diseases. Results: A fusion protein comprising the cysteine-rich region and parts of the flanking domains forms a trimer upon oxidization. Conclusion: The cysteine-rich region is an N-terminal cystine knot with novel topology. Significance: The cystine knot is also found in type IX collagen, indicating a general principle. Collagens are a group of extracellular matrix proteins with essential functions for skin integrity. Anchoring fibrils are made of type VII collagen (Col7) and link different skin layers together: the basal lamina and the underlying connective tissue. Col7 has a central collagenous domain and two noncollagenous domains located at the N and C terminus (NC1 and NC2), respectively. A cysteine-rich region of hitherto unknown function is located at the transition of the NC1 domain to the collagenous domain. A synthetic model peptide of this region was investigated by CD and NMR spectroscopy. The peptide folds into a collagen triple helix, and the cysteine residues form disulfide bridges between the different strands. The eight cystine knot topologies that are characterized by exclusively intermolecular disulfide bridges have been analyzed by molecular modeling. Two cystine knots are energetically preferred; however, all eight disulfide bridge arrangements are essentially possible. This novel cystine knot is present in type IX collagen, too. The conserved motif of the cystine knot is CX3CP. The cystine knot is N-terminal to the collagen triple helix in both collagens and therefore probably impedes unfolding of the collagen triple helix from the N terminus.