Flow cytometry assay for the detection of single-copy DNA in human lymphocytes

被引：2
作者：
Uno, Naoki [1 ]
Kaku, Norihito [1 ]
Morinaga, Yoshitomo [1 ]
Hasegawa, Hiroo [1 ]
Yanagihara, Katsunori [1 ]
机构：
[1] Nagasaki Univ, Dept Lab Med, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
来源：
NUCLEIC ACIDS RESEARCH | 2020年 / 48卷 / 15期
关键词：
IN-SITU HYBRIDIZATION; MESSENGER-RNA; NUCLEOTIDE POLYMORPHISMS; CELLS; PCR; AMPLIFICATION; PROVIRUS;
D O I：
10.1093/nar/gkaa515
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization, flow cytometric detection of specific genomic DNA sequences has not been established. Here, we present a flow cytometry assay for detection of single-copy genomic sequences in human lymphocytes using in situ PCR with universal energy transfer-labelled primers.
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页数：7
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