Flow cytometry assay for the detection of single-copy DNA in human lymphocytes

被引:2
作者
Uno, Naoki [1 ]
Kaku, Norihito [1 ]
Morinaga, Yoshitomo [1 ]
Hasegawa, Hiroo [1 ]
Yanagihara, Katsunori [1 ]
机构
[1] Nagasaki Univ, Dept Lab Med, Grad Sch Biomed Sci, Nagasaki 8528501, Japan
关键词
IN-SITU HYBRIDIZATION; MESSENGER-RNA; NUCLEOTIDE POLYMORPHISMS; CELLS; PCR; AMPLIFICATION; PROVIRUS;
D O I
10.1093/nar/gkaa515
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization, flow cytometric detection of specific genomic DNA sequences has not been established. Here, we present a flow cytometry assay for detection of single-copy genomic sequences in human lymphocytes using in situ PCR with universal energy transfer-labelled primers.
引用
收藏
页数:7
相关论文
共 50 条
  • [41] Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures
    Wiklander, Oscar P. B.
    Bostancioglu, R. Beklem
    Welsh, Joshua A.
    Zickler, Antje M.
    Murke, Florian
    Corso, Giulia
    Felldin, Ulrika
    Hagey, Daniel W.
    Evertsson, Bjorn
    Liang, Xiu-Ming
    Gustafsson, Manuela O.
    Mohammad, Dara K.
    Wiek, Constanze
    Hanenberg, Helmut
    Bremer, Michel
    Gupta, Dhanu
    Bjornstedt, Mikael
    Giebel, Bernd
    Nordin, Joel Z.
    Jones, Jennifer C.
    EL Andaloussi, Samir
    Goergens, Andre
    FRONTIERS IN IMMUNOLOGY, 2018, 9
  • [42] Minimal residual disease detection in multiple myeloma: comparison between BML single-tube 10-color multiparameter flow cytometry and EuroFlow multiparameter flow cytometry
    Sato, Kota
    Okazuka, Kiyoshi
    Ishida, Tadao
    Sakamoto, Jun
    Kaneko, Shigeto
    Nashimoto, Junichiro
    Uto, Yui
    Ogura, Mizuki
    Yoshiki, Yumiko
    Abe, Yu
    Maeda, Aki
    Hamazaki, Hiroyuki
    Tsukada, Nobuhiro
    Hiragohri, Yuji
    Suzuki, Kenshi
    ANNALS OF HEMATOLOGY, 2021, 100 (12) : 2989 - 2995
  • [43] High-Throughput Human Telomere Length Analysis at the Single-Chromosome Level by FISH Coupled with Nano-Flow Cytometry
    Gao, Kaimin
    Zhou, Yingxing
    Lu, Quanyi
    Lu, Jingyuan
    Su, Liyun
    Su, Rui
    Zhang, Miaomiao
    Tian, Ye
    Wu, Lina
    Yan, Xiaomei
    ANALYTICAL CHEMISTRY, 2021, 93 (27) : 9531 - 9540
  • [44] Single-copy sensitive, field-deployable, and simultaneous dual-gene detection of SARS-CoV-2 RNA via modified RT-RPA
    Xia, Simin
    Chen, Xi
    CELL DISCOVERY, 2020, 6 (01)
  • [45] An ex vivo multiparametric flow cytometry assay using human whole blood to simultaneously measure cytotoxicity and leishmanicidal activities
    Ribeiro, Juliana M.
    Bandeira, Cristiano C.
    de Faria, Bruno G.
    Alves, Marina L. R.
    Vieira, Francisco O.
    Giunchetti, Rodolfo C.
    Uzonna, Jude E.
    Teixeira-Carvalho, Andrea
    Peruhype-Magalhaes, Vanessa
    Souza-Fagundes, Elaine M.
    EXPERIMENTAL PARASITOLOGY, 2020, 216
  • [46] Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation
    Miklos, Morana
    Gajski, Goran
    Garaj-Vrhovac, Vera
    RADIOLOGY AND ONCOLOGY, 2009, 43 (02) : 97 - 107
  • [47] DNA repair capacity of cultured human lymphocytes exposed to mutagens measured by the comet assay and array expression analysis
    Bausinger, Julia
    Speit, Guenter
    MUTAGENESIS, 2015, 30 (06) : 811 - 820
  • [48] Assessing the performance of methods for copy number aberration detection from single-cell DNA sequencing data
    Mallory, Xian F.
    Edrisi, Mohammadamin
    Navin, Nicholas
    Nakhleh, Luay
    PLOS COMPUTATIONAL BIOLOGY, 2020, 16 (07)
  • [49] Detection of intracellular interleukin-8 in human mast cells: Flow cytometry as a guide for immunoelectron microscopy
    Grutzkau, A
    KrugerKrasagakes, S
    Kogel, H
    Moller, A
    Lippert, U
    Henz, BM
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1997, 45 (07) : 935 - 945
  • [50] Detection and Quantitation of DNA Mutations Based on Gold Magnetic Nanocomposites and Single Primer Extension Assay
    Liu, Ming
    Yang, Haowen
    Jiang, Hongrong
    He, Nongyue
    Zeng, Xin
    NANOSCIENCE AND NANOTECHNOLOGY LETTERS, 2019, 11 (02) : 214 - 221