Genome-wide mapping and analysis of chromosome architecture

被引：237

作者：

Schmitt, Anthony D. ^{[1
,2
]}

Hu, Ming ^{[3
,4
]}

Ren, Bing ^{[5
,6
]}

机构：

[1] Ludwig Inst Canc Res, 9500 Gilman Dr, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Biomed Sci Grad Program, 9500 Gilman Dr, La Jolla, CA 92093 USA

[3] NYU, Sch Med, Div Biostat, Dept Populat Hlth, 650 First Ave,Room 540, New York, NY 10016 USA

[4] Cleveland Clin Fdn, Lerner Res Inst, Dept Quantitat Hlth Sci, 9500 Euclid Ave, Cleveland, OH 44195 USA

[5] Univ Calif San Diego, Moores Canc Ctr, Dept Cellular & Mol Med, Ludwig Inst Canc Res, 9500 Gilman Dr, La Jolla, CA 92093 USA

[6] Univ Calif San Diego, Inst Genom Med, 9500 Gilman Dr, La Jolla, CA 92093 USA

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2016年 / 17卷 / 12期

基金：

美国国家卫生研究院;

关键词：

HI-C DATA; CONFORMATION CAPTURE ASSAYS; LONG-RANGE INTERACTIONS; CHROMATIN INTERACTIONS; HIGH-RESOLUTION; PROXIMITY-LIGATION; GENE-EXPRESSION; BIOCONDUCTOR PACKAGE; TOPOLOGICAL DOMAINS; LEVEL DECONVOLUTION;

D O I：

10.1038/nrm.2016.104

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Chromosomes of eukaryotes adopt highly dynamic and complex hierarchical structures in the nucleus. The three-dimensional (3D) organization of chromosomes profoundly affects DNA replication, transcription and the repair of DNA damage. Thus, a thorough understanding of nuclear architecture is fundamental to the study of nuclear processes in eukaryotic cells. Recent years have seen rapid proliferation of technologies to investigate genome organization and function. Here, we review experimental and computational methodologies for 3D genome analysis, with special focus on recent advances in high-throughput chromatin conformation capture (3C) techniques and data analysis.

引用

页码：743 / 755

页数：13

共 119 条

[1] Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition [J].