Klf10 inhibits IL-12p40 production in macrophage colony-stimulating factor-induced mouse bone marrow-derived macrophages

被引:20
作者
Zhang, Wei [1 ]
Wang, Xuelian [1 ]
Xia, Xiaoping [2 ]
Liu, Xia [1 ]
Suo, Shanshan [1 ]
Guo, Jing [1 ]
Li, Min [1 ]
Cao, Wenqiang [1 ]
Cai, Zhijian [1 ]
Hui, Zhaoyuan [1 ]
Subramaniam, Malayannan [3 ]
Spelsberg, Thomas C. [3 ]
Wang, Jianli [1 ]
Wang, Lie [1 ]
机构
[1] Zhejiang Univ, Sch Med, Inst Immunol, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Med, Clin Lab, Sir Run Run Shaw Hosp, Hangzhou 310058, Zhejiang, Peoples R China
[3] Mayo Clin, Dept Biochem & Mol Biol, Rochester, MN USA
基金
中国国家自然科学基金; 国家教育部博士点专项基金资助;
关键词
IL-12p40; Inflammatory factor; Klf10; Macrophage; Transcription factor; KRUPPEL-LIKE FACTOR; TRANSCRIPTION FACTOR; GM-CSF; TRANSFORMING GROWTH-FACTOR-BETA-1; MONOCYTE DIFFERENTIATION; INNATE RESISTANCE; ADAPTIVE IMMUNITY; INTERFERON-GAMMA; GENE INDUCTION; TGF-BETA;
D O I
10.1002/eji.201242697
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Bone marrow-derived macrophages (BMMs) treated with granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage colony-stimulating factor (M-CSF), differentiate into GM-CSF-induced mouse bone marrow-derived macrophages (GM-BMMs) or M-CSF-induced mouse bone marrow-derived macrophages (M-BMMs), which have an M1 or M2 profile, respectively. GM-BMMs produce large amounts of proinflammatory cytokines and mediate resistance to pathogens, whereas M-BMMs produce antiinflammatory cytokines that contribute to tissue repair and remodeling. M-BMMs stimulated with lipopolysaccharide (LPS) are in an antiinflammatory state, with an IL-12lowIL-10high phenotype. However, the regulation of this process remains unclear. Klf10 belongs to the family of Kruppel-like transcription factors and was initially described as a TGF-beta inducible early gene 1. IL-12p40 is upregulated in LPS-stimulated M-BMMs from Klf10-deficient mice, but downregulated during Klf10 overexpression. Klf11, another member of the Kruppel-like factor family, can also repress the production of IL-12p40. Furthermore, Klf10 binds to the CACCC element of the IL-12p40 promoter and inhibits its transcription. We have therefore identified Klf10 as a transcription factor that regulates the expression of IL-12p40 in M-BMMs.
引用
收藏
页码:258 / 269
页数:12
相关论文
共 46 条
[1]   Kruppel-like factor 4 is essential for inflammatory monocyte differentiation in vivo [J].
Alder, Jonathan K. ;
Georgantas, Robert W., III ;
Hildreth, Richard L. ;
Kaplan, Ian M. ;
Morisot, Sebastien ;
Yu, Xiaobing ;
McDevitt, Michael ;
Civin, Curt I. .
JOURNAL OF IMMUNOLOGY, 2008, 180 (08) :5645-5652
[2]  
Aste-Amezaga M, 1998, J IMMUNOL, V160, P5936
[3]  
Bastos KRB, 2002, J LEUKOCYTE BIOL, V71, P271
[4]   MODULATION OF THE ANTILISTERIAL ACTIVITY OF HUMAN BLOOD-DERIVED MACROPHAGES BY ACTIVATING AND DEACTIVATING CYTOKINES [J].
BLAUER, F ;
GROSCURTH, P ;
SCHNEEMANN, M ;
SCHOEDON, G ;
SCHAFFNER, A .
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1995, 15 (02) :105-114
[5]   Role of Kruppel-like factors in leukocyte development, function, and disease [J].
Cao, Zhuoxiao ;
Sun, Xinghui ;
Icli, Basak ;
Wara, Akm Khyrul ;
Feinberg, Mark W. .
BLOOD, 2010, 116 (22) :4404-4414
[6]   Kruppel-like Factor KLF10 Targets Transforming Growth Factor-β1 to Regulate CD4+CD25- T Cells and T Regulatory Cells [J].
Cao, Zhuoxiao ;
Wara, Akm Khyrul ;
Icli, Basak ;
Sun, Xinghui ;
Packard, Rene R. S. ;
Esen, Fehim ;
Stapleton, Christopher J. ;
Subramaniam, Malayannan ;
Kretschmer, Karsten ;
Apostolou, Irina ;
von Boehmer, Harald ;
Hansson, Goran K. ;
Spelsberg, Thomas C. ;
Libby, Peter ;
Feinberg, Mark W. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (37) :24914-24924
[7]   Unanticipated repression function linked to erythroid Kruppel-like factor [J].
Chen, XY ;
Bieker, JJ .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (09) :3118-3125
[8]   Two roads diverged:: Interferon α/β- and interleukin 12-mediated pathways in promoting T cell interferon γ responses during viral infection [J].
Cousens, LP ;
Peterson, R ;
Hsu, S ;
Dorner, A ;
Altman, JD ;
Ahmed, R ;
Biron, CA .
JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (08) :1315-1327
[9]   Mechanism of inhibition of LPS-induced IL-12p40 production by IL-10 and TGF-β in ANA-1 cells [J].
Du, CG ;
Sriram, S .
JOURNAL OF LEUKOCYTE BIOLOGY, 1998, 64 (01) :92-97
[10]   The Kruppel-like factor KLF4 is a critical regulator of monocyte differentiation [J].
Feinberg, Mark W. ;
Wara, Akm Khyrul ;
Cao, Zhuoxiao ;
Lebedeva, Maria A. ;
Rosenbauer, Frank ;
Iwasaki, Hiromi ;
Hirai, Hideyo ;
Katz, Jonathan P. ;
Haspel, Richard L. ;
Gray, Susan ;
Akashi, Koichi ;
Segre, Julie ;
Kaestner, Klaus H. ;
Tenen, Daniel G. ;
Jain, Mukesh K. .
EMBO JOURNAL, 2007, 26 (18) :4138-4148