Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris

被引：0

作者：

Burowa, Susann ^{[1
]}

Fontaine, Romain ^{[1
]}

von Krogh, Kristine ^{[1
]}

Mayer, Ian ^{[2
]}

Nourizadeh-Lillabadi, Rasoul ^{[1
]}

Hollander-Cohen, Lian ^{[3
]}

Cohen, Yaron ^{[3
]}

Shpilman, Michal ^{[3
]}

Levavi-Sivan, Berta ^{[3
]}

Weltzien, Finn-Arne ^{[1
]}

机构：

[1] Norwegian Univ Life Sci, Dept Basic Sci & Aquat Med, Fac Vet Med, N-0454 Oslo, Norway

[2] Norwegian Univ Life Sci, Dept Prod Anim Clin Sci, Fac Vet Med, N-0454 Oslo, Norway

[3] Hebrew Univ Jerusalem, Dept Anim Sci, Fac Agr Food & Environm, IL-76100 Rehovot, Israel

来源：

DATA IN BRIEF | 2019年 / 22卷

关键词：

Enzyme-linked immunosorbent assay; Follicle-stimulating hormone; Luteinizing hormone; Oryzias latipes; Recombinant gonadotropins; Western blot analysis; TILAPIA;

D O I：

10.1016/j.dib.2019.01.034

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fsh beta (mdFsh beta), Lh beta (mdLh beta), Fsh beta alpha (mdFsh beta alpha), and Lh beta alpha (mdLh beta alpha) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fsh beta and Lh beta by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshpoi and mdLh beta alpha within enzyme-linked immunosorbent assays (ELISA5), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled "Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression" (Burow et al., in press). (C) 2019 Published by Elsevier Inc.

引用

页码：1057 / 1063

页数：7

共 4 条

[1] Development of specific enzyme-linked immunosorbent assay for determining LH and FSH levels in tilapia, using recombinant gonadotropins
Aizen, Joseph
Kasuto, Harel
Levavi-Sivan, Berta
[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2007, 153 (1-3) : 323 - 332
[2] Medaka follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression levels
Burow, Susann
Fontaine, Romain
von Krogh, Kristine
Mayer, Ian
Nourizadeh-Lillabadi, Rasoul
Hollander-Cohen, Lian
Cohen, Yaron
Shpilman, Michal
Levavi-Sivan, Berta
Weltzien, Finn-Arne
[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2019, 272 : 93 - 108
[3] Production of biologically active tethered tilapia LHβα by the methylotrophic yeast Pichia pastoris
Kasuto, H
Levavi-Sivan, B
[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2005, 140 (03) : 222 - 232
[4] Gonadotropins in the Russian Sturgeon: Their Role in Steroid Secretion and the Effect of Hormonal Treatment on Their Secretion
Yom-Din, Svetlana
Hollander-Cohen, Lian
Aizen, Joseph
Boehm, Benjamin
Shpilman, Michal
Golan, Matan
Hurvitz, Avshalom
Degani, Gad
Levavi-Sivan, Berta
[J]. PLOS ONE, 2016, 11 (09):

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