Surface display of enzyme complex on Corynebacterium glutamicum as a whole cell biocatalyst and its consolidated bioprocessing using fungal-pretreated lignocellulosic biomass

被引：5

作者：

Lee, Myeong-Eun ^{[1
]}

Ko, Young Jin ^{[1
,2
]}

Hwang, Dong-Hyeok ^{[1
]}

Cho, Byeong-Hyeon ^{[1
]}

Jeong, Wu-Young ^{[1
]}

Bhardwaj, Nisha ^{[1
]}

Han, Sung Ok ^{[1
]}

机构：

[1] Korea Univ, Dept Biotechnol, Seoul 02841, South Korea

[2] Korea Univ, Inst Life Sci & Nat Resources, Seoul 02841, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 362卷

基金：

新加坡国家研究基金会;

关键词：

N-Acetylglucosaminidase; Cellulase; Cellulosome; Isopropanol; Corynebacterium glutamicum; IRPEX-LACTEUS; CLOSTRIDIUM-CELLULOVORANS; BIOLOGICAL PRETREATMENT; FERMENTATION; DEGRADATION; HYDROLYSIS; SYSTEM;

D O I：

10.1016/j.biortech.2022.127758

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A novel whole cell biocatalyst using fungal-pretreated lignocellulosic biomass was developed by displaying the enzyme complex consisting of N-acetylglucosaminidase (cNAG) and endoglucanse E (cCelE) on Corynebacterium glutamicum, hereafter called mNC. mNC showed a maximum 4.43-fold cNAG and 2.40-fold cCelE activity compared to single enzyme-secreting C. glutamicum. mNC also showed the highest efficiency of sugar production in various types of cellulose and fungal-pretreated biomass. The growth of mNC was 5.06-fold higher than that of the control. Then, the ability of mNC to produce a valuable chemical was confirmed. mNC overexpressing isopropanol biosynthesis genes showed a maximum titer of 218.9 +/- 11.73 mg/L isopropanol and maintained high efficiency for isopropanol production in the recycling test, which was 90.07 +/- 4.12 % during 4 cycles. This strategy can be applied to the direct saccharification of fungal-pretreated lignocellulosic biomass efficiently leading to the production of valuable products in various industrial fields.

引用

页数：8

共 2 条

[1] Cell Surface Display of Yarrowia lipolytica Lipase Lip2p Using the Cell Wall Protein YlPir1p, Its Characterization, and Application as a Whole-Cell Biocatalyst
Yuzbasheva, Evgeniya Y.
Yuzbashev, Tigran V.
Perkovskaya, Natalia I.
Mostova, Elizaveta B.
Vybornaya, Tatiana V.
Sukhozhenko, Aleksei V.
Toropygin, Ilya Y.
Sineoky, Sergey P.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 175 (08) : 3888 - 3900
[2] Cell Surface Display of Yarrowia lipolytica Lipase Lip2p Using the Cell Wall Protein YlPir1p, Its Characterization, and Application as a Whole-Cell Biocatalyst
Evgeniya Y. Yuzbasheva
Tigran V. Yuzbashev
Natalia I. Perkovskaya
Elizaveta B. Mostova
Tatiana V. Vybornaya
Aleksei V. Sukhozhenko
Ilya Y. Toropygin
Sergey P. Sineoky
Applied Biochemistry and Biotechnology, 2015, 175 : 3888 - 3900

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