Surface display of enzyme complex on Corynebacterium glutamicum as a whole cell biocatalyst and its consolidated bioprocessing using fungal-pretreated lignocellulosic biomass

被引：5

作者：

Lee, Myeong-Eun ^{[1
]}

Ko, Young Jin ^{[1
,2
]}

Hwang, Dong-Hyeok ^{[1
]}

Cho, Byeong-Hyeon ^{[1
]}

Jeong, Wu-Young ^{[1
]}

Bhardwaj, Nisha ^{[1
]}

Han, Sung Ok ^{[1
]}

机构：

[1] Korea Univ, Dept Biotechnol, Seoul 02841, South Korea

[2] Korea Univ, Inst Life Sci & Nat Resources, Seoul 02841, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 362卷

基金：

新加坡国家研究基金会;

关键词：

N-Acetylglucosaminidase; Cellulase; Cellulosome; Isopropanol; Corynebacterium glutamicum; IRPEX-LACTEUS; CLOSTRIDIUM-CELLULOVORANS; BIOLOGICAL PRETREATMENT; FERMENTATION; DEGRADATION; HYDROLYSIS; SYSTEM;

D O I：

10.1016/j.biortech.2022.127758

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A novel whole cell biocatalyst using fungal-pretreated lignocellulosic biomass was developed by displaying the enzyme complex consisting of N-acetylglucosaminidase (cNAG) and endoglucanse E (cCelE) on Corynebacterium glutamicum, hereafter called mNC. mNC showed a maximum 4.43-fold cNAG and 2.40-fold cCelE activity compared to single enzyme-secreting C. glutamicum. mNC also showed the highest efficiency of sugar production in various types of cellulose and fungal-pretreated biomass. The growth of mNC was 5.06-fold higher than that of the control. Then, the ability of mNC to produce a valuable chemical was confirmed. mNC overexpressing isopropanol biosynthesis genes showed a maximum titer of 218.9 +/- 11.73 mg/L isopropanol and maintained high efficiency for isopropanol production in the recycling test, which was 90.07 +/- 4.12 % during 4 cycles. This strategy can be applied to the direct saccharification of fungal-pretreated lignocellulosic biomass efficiently leading to the production of valuable products in various industrial fields.

引用

页数：8