Antiallergic Asthma Properties of Brazilin through Inhibition of TH2 Responses in T Cells and in a Murine Model of Asthma

被引：26

作者：

Lee, Chen-Chen ^{[1
,2
]}

Wang, Chien-Neng ^{[2
]}

Kang, Jaw-Jou ^{[3
]}

Liao, Jiunn-Wang ^{[4
]}

Chiang, Bor-Luen ^{[5
]}

Chen, Hui-Chen ^{[2
]}

Hu, Chien-Ming ^{[6
]}

Lin, Chia-Der ^{[7
]}

Huang, Shih-Hsuan ^{[3
]}

Lai, Yu-Ting ^{[8
]}

机构：

[1] China Med Univ, Coll Med, Sch Med, Dept Microbiol & Immunol, Taichung, Taiwan

[2] China Med Univ, Coll Med, Grad Inst Basic Med Sci, Taichung, Taiwan

[3] Natl Taiwan Univ, Coll Med, Inst Toxicol, Taipei 10764, Taiwan

[4] Natl Chung Hsing Univ, Coll Vet Med, Grad Inst Vet Pathol, Taichung 40227, Taiwan

[5] Natl Taiwan Univ, Coll Med, Dept Clin Med, Taipei 10764, Taiwan

[6] Taipei Med Univ Hosp, Dept Emergency, Taipei, Taiwan

[7] China Med Univ, Sch Med, Dept Otolaryngol, Taichung, Taiwan

[8] China Med Univ, Grad Inst Immunol, Taichung, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 37期

关键词：

brazilin; airway remodeling; allergic inflammation; GATA-3; INDUCED AIRWAY HYPERRESPONSIVENESS; NITRIC-OXIDE SYNTHASE; CAESALPINIA-SAPPAN; ENDOTHELIAL-CELLS; IL-5; PRODUCTION; EL-4; CELLS; C-MAF; INDUCTION; GATA-3; CONSTITUENTS;

D O I：

10.1021/jf2038778

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This study aimed to determine whether brazilin exhibits anti-inflammatory effects that inhibit T helper cell type II (T(H)2) responses and whether it suppresses allergic inflammation reactions in a murine model of asthma. We found that brazilin inhibited the mRNA and protein expression of interleukin (IL)-4 and IL-5 induced by phorbol myristate acetate (PMA) and cAMP in EL-4 T cells in a dose-dependent manner. Following the intratracheal instillation of brazilin in ovalbumin (OVA)immunized mice, we found that brazilin-treated mice exhibited decreases in the release of IL-4, IL-5, IL-13, eotaxin-1, and tumor necrosis factor-alpha in bronchoalveolar lavage fluid (BALF); inhibited T(H)2 functioning via a decrease in IL-4 production; and exhibited attenuation of OVA-induced lung eosinophilia, airway hyperresponsiveness, and airway remodeling. These results suggest that brazilin exhibits anti-T(H)2 effects both in vitro and in vivo and may possess therapeutic potential for allergic diseases.

引用

页码：9405 / 9414

页数：10

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