Peptide-laden mesoporous silica nanoparticles with promoted bioactivity and osteo-differentiation ability for bone tissue engineering

被引：60

作者：

Luo, Zuyuan ^{[1
,2
]}

Deng, Yi ^{[1
,2
]}

Zhang, Ranran ^{[3
]}

Wang, Mengke ^{[1
]}

Bai, Yanjie ^{[4
,5
]}

Zhao, Qiang ^{[4
,5
]}

Lyu, Yalin ^{[3
]}

Wei, Jie ^{[6
]}

Wei, Shicheng ^{[1
,2
]}

机构：

[1] Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100081, Peoples R China

[2] Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China

[3] Capital Med Univ, Beijing Anzhen Hosp, Dept Stomatol, Beijing 100029, Peoples R China

[4] China Med Univ, Aviat Gen Hosp, Dept Stomatol, Beijing 100012, Peoples R China

[5] Chinese Acad Sci, Being Inst Translat Med, Beijing 100012, Peoples R China

[6] E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 131卷

基金：

北京市自然科学基金;

关键词：

Mesoporous silica nanoparticles; Peptide; Bioactivity; Osteo-differentiation; Bone tissue engineering; MESENCHYMAL STEM-CELLS; COLLOIDAL GELS; NANOSTRUCTURED MATERIALS; DRUG-DELIVERY; SCAFFOLDS; RELEASE; REGENERATION; BMP-2; PROLIFERATION; DEXAMETHASONE;

D O I：

10.1016/j.colsurfb.2015.04.043

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Combination of mesoporous silica materials and bioactive factors is a promising niche-mimetic solution as a hybrid bone substitution for bone tissue engineering. In this work, we have synthesized biocompatible silica-based nanoparticles with abundant mesoporous structure, and incorporated bone-forming peptide (BFP) derived from bone morphogenetic protein-7 (BMP-7) into the mesoporous silica nanoparticles (MSNs) to obtain a slow-release system for osteogenic factor delivery. The chemical characterization demonstrates that the small osteogenic peptide is encapsulated in the mesoporous successfully, and the nitrogen adsorption-desorption isotherms suggest that the peptide encapsulation has no influence on mesoporous structure of MSNs. In the cell experiment, the peptide-laden MSNs (p-MSNs) show higher MG-63 cell proliferation, spreading and alkaline phosphatase (ALP) activity than the bare MSNs, indicating good in vitro cytocompatibility. Simultaneously, the osteogenesis-related proteins expression and calcium mineral deposition disclose enhanced osteo-differentiation of human mesenchymal stem cells (hMSCs) under the stimulation of the p-MSNs, confirming that BFP released from MSNs could significantly promote the osteogenic differentiation of hMSCs, especially at 500 mu g/mL of p-MSNs concentration. The peptide-modified MSNs with better bioactivity and osteogenic differentiation make it a potential candidate as bioactive material for bone repairing, bone regeneration, and bio-implant coating applications. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：73 / 82

页数：10

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