Exploiting natural chemical photosensitivity of anhydrotetracycline and tetracycline for dynamic and setpoint chemo-optogenetic control

被引:39
作者
Baumschlager, Armin [1 ]
Rullan, Marc [1 ]
Khammash, Mustafa [1 ]
机构
[1] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn D BSSE, Mattenstr 26, CH-4058 Basel, Switzerland
基金
欧盟地平线“2020”; 欧洲研究理事会;
关键词
GENE-EXPRESSION; ESCHERICHIA-COLI; TRANSCRIPTIONAL ACTIVATION; RNA-POLYMERASE; LIGHT; PHOTODEGRADATION; PHARMACEUTICALS; BIOLOGY; SYSTEMS; CELLS;
D O I
10.1038/s41467-020-17677-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The transcriptional inducer anhydrotetracycline (aTc) and the bacteriostatic antibiotic tetracycline (Tc) are commonly used in all fields of biology for control of transcription or translation. A drawback of these and other small molecule inducers is the difficulty of their removal from cell cultures, limiting their application for dynamic control. Here, we describe a simple method to overcome this limitation, and show that the natural photosensitivity of aTc/Tc can be exploited to turn them into highly predictable optogenetic transcriptional- and growth-regulators. This new optogenetic class uniquely features both dynamic and setpoint control which act via population-memory adjustable through opto-chemical modulation. We demonstrate this method by applying it for dynamic gene expression control and for enhancing the performance of an existing optogenetic system. We then expand the utility of the aTc system by constructing a new chemical bandpass filter that increases its aTc response range. The simplicity of our method enables scientists and biotechnologists to use their existing systems employing aTc/Tc for dynamic optogenetic experiments without genetic modification. Anhydrotetracycline and tetracycline are commonly used chemicals to regulate transcription and translation, respectively. Here the authors exploit the natural photosensitivity of these molecules to place their activity under optical control.
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页数:10
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[1]   A universal biomolecular integral feedback controller for robust perfect adaptation [J].
Aoki, Stephanie K. ;
Lillacci, Gabriele ;
Gupta, Ankit ;
Baumschlager, Armin ;
Schweingruber, David ;
Khammash, Mustafa .
NATURE, 2019, 570 (7762) :533-+
[2]   Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control [J].
Baumschlager, Armin ;
Aoki, Stephanie K. ;
Khammash, Mustafa .
ACS SYNTHETIC BIOLOGY, 2017, 6 (11) :2157-2167
[3]   Photocaged Arabinose: A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression [J].
Binder, Dennis ;
Bier, Claus ;
Gruenberger, Alexander ;
Drobietz, Dagmar ;
Hage-Huelsmann, Jennifer ;
Wandrey, Georg ;
Buechs, Jochen ;
Kohlheyer, Dietrich ;
Loeschcke, Anita ;
Wiechert, Wolfgang ;
Jaeger, Karl-Erich ;
Pietruszka, Joerg ;
Drepper, Thomas .
CHEMBIOCHEM, 2016, 17 (04) :296-299
[4]   Photodegradation of pharmaceuticals in the aquatic environment: A review [J].
Boreen, AL ;
Arnold, WA ;
McNeill, K .
AQUATIC SCIENCES, 2003, 65 (04) :320-341
[5]   Positively regulated bacterial expression systems [J].
Brautaset, Trygve ;
Lale, Rahmi ;
Valla, Svein .
MICROBIAL BIOTECHNOLOGY, 2009, 2 (01) :15-30
[6]   Dynamic metabolic engineering: New strategies for developing responsive cell factories [J].
Brockman, Irene M. ;
Prather, Kristala L. J. .
BIOTECHNOLOGY JOURNAL, 2015, 10 (09) :1360-1369
[7]   How to train your microbe: methods for dynamically characterizing gene networks [J].
Castillo-Hair, Sebastian M. ;
Igoshin, Oleg A. ;
Tabor, Jeffrey J. .
CURRENT OPINION IN MICROBIOLOGY, 2015, 24 :113-123
[8]   Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance [J].
Chopra, I ;
Roberts, M .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (02) :232-+
[9]   ONE-STEP PREPARATION OF COMPETENT ESCHERICHIA-COLI - TRANSFORMATION AND STORAGE OF BACTERIAL-CELLS IN THE SAME SOLUTION [J].
CHUNG, CT ;
NIEMELA, SL ;
MILLER, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2172-2175
[10]  
DEBOER HA, 1983, P NATL ACAD SCI-BIOL, V80, P21