Evaluation of the Abbott LCx quantitative assay for measurement of human immunodeficiency virus RNA in plasma

被引：22

作者：

de Mendoza, C

Alcamí, J

Sainz, M

Folgueira, D

Soriano, V

机构：

[1] Hosp Carlos III, Inst Salud Carlos III, Infect Dis Serv, Madrid, Spain

[2] Hosp Doce Octubre, Microbiol Serv, Madrid, Spain

[3] Abbott Labs, Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 04期

关键词：

D O I：

10.1128/JCM.40.4.1518-1521.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasma human immunodeficiency virus RNA in 491 clinical specimens was measured by LCx. There was a strong correlation with the results provided by other methods (r(2) values of 0.93 with Cobas-Monitor version 1.5 and of 0.95 with Quantiplex version 3.0). However, values were uniformly higher with LCx than with Quantiplex when non-B subtypes were tested.

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页码：1518 / 1521

页数：4

相关论文

共 14 条

[1] DETECTION OF DIVERSE HIV-1 GENETIC SUBTYPES IN THE USA [J].

BRODINE, SK ;

MASCOLA, JR ;

WEISS, PJ ;

ITO, SI ;

PORTER, KR ;

ARTENSTEIN, AW ;

GARLAND, FC ;

MCCUTCHAN, FE ;

BURKE, DS .

LANCET, 1995, 346 (8984) :1198-1199

[2] A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml [J].

Collins, ML ;

Irvine, B ;

Tyner, D ;

Fine, E ;

Zayati, C ;

Chang, CA ;

Horn, T ;

Ahle, D ;

Detmer, J ;

Shen, LP ;

Kolberg, J ;

Bushnell, S ;

Urdea, MS ;

Ho, DD .

NUCLEIC ACIDS RESEARCH, 1997, 25 (15) :2979-2984

[3]

Holguín A, 1999, J MED VIROL, V59, P189, DOI 10.1002/(SICI)1096-9071(199910)59:2&lt

[4]

189::AID-JMV10&gt

[5]

3.0.CO

[6]

2-A

[7] Protease gene analysis of HIV type 1 non-B subtypes in Spain [J].

Holguín, A ;

Rodés, B ;

Soriano, V .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 2000, 16 (14) :1395-1403

[8] Erroneously low or undetectable plasma human immunodeficiency virus type 1 (HIV-1) ribonucleic acid load determined by polymerase chain reaction, in West African and American patients with non-B subtype HIV-1 infection [J].

Jenny-Avital, ER ;

Beatrice, ST .

CLINICAL INFECTIOUS DISEASES, 2001, 32 (08) :1227-1230

[9] Equivalent amplification of intrinsically variable nucleic acid sequences by multiple-primer-induced overlapping amplification assay: Applications for universal detection and quantitation [J].

Lu, W ;

Cao, L ;

Ty, L ;

Arlie, M ;

Andrieu, JM .

NATURE MEDICINE, 1999, 5 (09) :1081-1085

[10] Phylogeny of HIV type 1 group O isolates based on env gene sequences [J].

Mas, A ;

Quiñones-Mateu, ME ;

Domingo, E ;

Soriano, V .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1999, 15 (08) :769-773