Effects of single-walled carbon nanotubes on the functions of plasma proteins and potentials in vascular prostheses

被引：28

作者：

Meng, Jie ^{[1
,2
]}

Song, Li ^{[3
]}

Xu, Haiyan ^{[1
,2
]}

Kong, Hua ^{[1
,2
]}

Wang, Chaoying ^{[3
]}

Guo, Xiaotian ^{[1
,2
]}

Xie, Sishen ^{[3
]}

机构：

[1] Chinese Acad Med Sci, Inst Basic Med, Beijing, Peoples R China

[2] Peking Union Med Coll, Beijing, Peoples R China

[3] Chinese Acad Sci, Inst Phys, Beijing, Peoples R China

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2005年 / 1卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Carbon nanotubes; Protein; Adsorption behaviors; Platelets; Implantable biomaterials;

D O I：

10.1016/j.nano.2005.03.003

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Background: Nonwoven single-walled nanotubes (SWNTs) are novel materials with a nanotopography macroscopic surface and pure-carbon composition, which may serve as coatings on implants in blood-contact environments. Methods: The adsorption behavior of albumin, fibrinogen, and fresh plasma on SWNT membranes was studied using scanning electron microscopy (SEM), energy-dispersive x-ray, and direct enzymelinked immunosorbent assay, respectively. Platelet adhesion and activation were investigated in vitro using SEM observation, and via flow cytometry analysis by PAC-1, which binds to GPIIb/IIIa expressed on activated platelets. Results: Nonwoven SWNTs clearly displayed greater adsorption preference for fibrinogen than for albumin. However, adhered platelets were not observed by SEM. Results obtained from flow cytometry analysis further proved very low expression of GPIIb/ IIIa on platelets caused by nonwoven SWNTs. Conclusion: The function of adsorbed fibrinogen to mediate platelet recognition, adhesion, activation, and aggregation was significantly suppressed, which induced extremely low levels of platelet adhesion and activation. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：136 / 142

页数：7

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