Exploring Quaternized Hydroxyethylcellulose as Potential Gene Carriers

被引：2

作者：

Ding Fei ^{[1
]}

You Jun ^{[1
]}

Weng Xiaocheng ^{[1
]}

Zhou Jinping ^{[1
]}

Zhang Xiaolian ^{[2
]}

Zhou Xiang ^{[1
,3
]}

Zhang Lina ^{[1
]}

机构：

[1] Wuhan Univ, State Key Lab Virol, Coll Chem & Mol Sci, Key Lab Biomed Polymers,Minist Educ, Wuhan 430072, Hubei, Peoples R China

[2] Wuhan Univ, Sch Med, Wuhani 430071, Hubei, Peoples R China

[3] Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China

来源：

CHINESE JOURNAL OF CHEMISTRY | 2012年 / 30卷 / 09期

基金：

中国国家自然科学基金;

关键词：

cationic cellulose; DNA; gene carrier; transfection; IN-VITRO; PLASMID DNA; DELIVERY; CELLULOSE; NANOPARTICLES; EXPRESSION; CHITOSAN; THERAPY; POLYMERS; VECTOR;

D O I：

10.1002/cjoc.201200659

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose (QHEC) derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter (DLS) results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfection abilities in comparison to the naked DNA. The cytotoxicities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low compared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as potential gene delivery vectors.

引用

页码：2212 / 2218

页数：7

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