Assessment of DNA damage induced in vitro by etoposide and two fungicides (carbendazim and chlorothalonil) in human lymphocytes with the comet assay

被引:67
作者
Lebailly, P
Vigreux, C
Godard, T
Sichel, F
Bar, E
LeTalaer, JY
HenryAmar, M
Gauduchon, P
机构
[1] UNIV CAEN, EA 1772, F-14032 CAEN, FRANCE
[2] CTR FRANCOIS BACLESSE, EXPT CANCEROL LAB, F-14021 CAEN, FRANCE
[3] CTR FRANCOIS BACLESSE, REGISTRE GEN TUMEURS CALVADOS, F-14021 CAEN, FRANCE
关键词
comet assay; SCGE assay; human lymphocyte; fungicide; pesticide; etoposide; DNA damage; cell viability;
D O I
10.1016/S0027-5107(97)00015-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effects of two fungicides (carbendazim and chlorothalonil) on the induction of DNA damage in human peripheral blood lymphocytes (human PBL) have been investigated using the single cell gel electrophoresis assay (SCGE assay or comet assay) immediately after a 1-h treatment and after a 24-h post-treatment incubation. The assessment of etoposide (an effective antitumour agent) effects on human PBL in terms of cell viability and dose-DNA damage relationships was made and etoposide selected as a positive control. The results indicate that etoposide induces significant(p < 0.01) dose-dependent DNA damages for concentrations at which the loss of cell viability is low. After a 24-h recuperation period, all observed DNA damages had disappeared. With SCGE assay performed after a 1-h treatment, similar positive results were observed with chlorothalonil alone or in association with carbendazim, without any loss of cell viability. However, a dramatic loss of cell viability was measured after 24 h and was associated with a large proportion of highly damaged cells. In contrast, carbendazim was not cytotoxic on human PBL and did not induced DNA damage using the SCGE assay either immediately after treatment or after a 24-h post-treatment incubation. These results point to the necessity of an adequate evaluation of immediate and long-term cytotoxicity of compounds that are to be assessed by the SCGE assay.
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页码:205 / 217
页数:13
相关论文
共 35 条
[1]  
[Anonymous], 1992, JOINT M FAO PAN EXP
[2]  
BESSI H, 1994, CANCER J - FRANCE, V7, P248
[3]   CANCER AMONG FARMERS - A REVIEW [J].
BLAIR, A ;
MALKER, H ;
CANTOR, KP ;
BURMEISTER, L ;
WIKLUND, K .
SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH, 1985, 11 (06) :397-407
[4]   CYTOGENETIC BIOMONITORING IN A SPANISH GROUP OF AGRICULTURAL-WORKERS EXPOSED TO PESTICIDES [J].
CARBONELL, E ;
XAMENA, N ;
CREUS, A ;
MARCOS, R .
MUTAGENESIS, 1993, 8 (06) :511-517
[5]   A SURVEY OF EPA/OPP AND OPEN LITERATURE DATA ON SELECTED PESTICIDE CHEMICALS TESTED FOR MUTAGENICITY .1. INTRODUCTION AND 1ST 10 CHEMICALS [J].
DEARFIELD, KL ;
STACK, HF ;
QUEST, JA ;
WHITING, RJ ;
WATERS, MD .
MUTATION RESEARCH, 1993, 297 (03) :197-233
[6]   CYTOGENETIC BIOMONITORING OF AN ITALIAN POPULATION EXPOSED TO PESTICIDES - CHROMOSOME ABERRATION AND SISTER-CHROMATID EXCHANGE ANALYSIS IN PERIPHERAL-BLOOD LYMPHOCYTES [J].
DEFERRARI, M ;
ARTUSO, M ;
BONASSI, S ;
BONATTI, S ;
CAVALIERI, Z ;
PESCATORE, D ;
MARCHINI, E ;
PISANO, V ;
ABBONDANDOLO, A .
MUTATION RESEARCH, 1991, 260 (01) :105-113
[7]  
*EU, 1993, OFF J EUR COMMUN
[8]   THE COMET ASSAY - A COMPREHENSIVE REVIEW [J].
FAIRBAIRN, DW ;
OLIVE, PL ;
ONEILL, KL .
MUTATION RESEARCH-REVIEWS IN GENETIC TOXICOLOGY, 1995, 339 (01) :37-59
[9]   GENOTOXICITY OF DITHIOCARBAMATES AND THEIR METABOLITES [J].
FRANEKIC, J ;
BRATULIC, N ;
PAVLICA, M ;
PAPES, D .
MUTATION RESEARCH LETTERS, 1994, 325 (2-3) :65-74
[10]   EVALUATION OF THE GENETIC-ACTIVITY PROFILES OF 65 PESTICIDES [J].
GARRETT, NE ;
STACK, HF ;
WATERS, MD .
MUTATION RESEARCH, 1986, 168 (03) :301-325