Current practice of liquid chromatography-mass spectrometry in metabolomics and metabonomics

被引：314

作者：

Gika, Helen G. ^{[1
]}

Theodoridis, Georgios A. ^{[2
]}

Plumb, Robert S. ^{[3
]}

Wilson, Ian D. ^{[3
]}

机构：

[1] Aristotle Univ Thessaloniki, Dept Chem Engn, Thessaloniki 54124, Greece

[2] Aristotle Univ Thessaloniki, Dept Chem, Thessaloniki 54124, Greece

[3] Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Surg & Canc, Sect Computat & Syst Med, London SW7 2AY, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2014年 / 87卷

关键词：

Metabolomics; Metabonomics; Disease biomarker discovery; LC-MS; Metabolic profiling; HUMAN URINE; HYDROPHILIC INTERACTION; UPLC-MS; TOF-MS; QUANTITATIVE-ANALYSIS; METABOLITE PROFILES; SAMPLE PREPARATION; CANCER-DETECTION; SYSTEMS BIOLOGY; HUMAN PLASMA;

D O I：

10.1016/j.jpba.2013.06.032

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on publication and citation numbers liquid chromatography (LC-MS) has become the major analytical technology in the field of global metabolite profiling. This dominance reflects significant investments from both the research community and instrument manufacturers. Here an overview of the approaches taken for LC-MS-based metabolomics research is given, describing critical steps in the realisation of such studies: study design and its needs, specific technological problems to be addressed and major obstacles in data treatment and biomarker identification. The current state of the art for LC-MS-based analysis in metabonomics/metabolomics is described including recent developments in liquid chromatography, mass spectrometry and data treatment as these are applied in metabolomics underlining the challenges, limitations and prospects for metabolomics research. Examples of the application of metabolite profiling in the life sciences focusing on disease biomarker discovery are highlighted. In addition, new developments and future prospects are described. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：12 / 25

页数：14

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