Increased 8-isoprostane, a marker of oxidative stress in exhaled breath condensate in subjects with asbestos exposure

被引：55
作者：
Pelclova, Daniela [1 ]
Fenclova, Zdenka [1 ]
Kacer, Petr [2 ]
Kuzma, Marek [3 ]
Navratil, Tomas [4 ]
Lebedova, Jindriska [1 ]
机构：
[1] Charles Univ Prague, Dept Occupat Med, Fac Med 1, Prague 12000, Czech Republic
[2] Inst Chem Technol, CR-16628 Prague 6, Czech Republic
[3] AS CR, Inst Microbiol, Prague 14220 4, Czech Republic
[4] AS CR, J Heyrovsky Inst Phys Chem, Prague 18223 8, Czech Republic
来源：
INDUSTRIAL HEALTH | 2008年 / 46卷 / 05期
关键词：
asbestos; asbestosis; pleural hyalinosis; 8-isoprostane; breath condensate; rheumatoid factor; alpha; 1-microglobulin; 1-antitrypsin; cotinine;
D O I：
10.2486/indhealth.46.484
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Asbestosis and pleural plaques exhibit unpredictable but progressive development, and there are no markers routinely available to measure their prognosis. Asbestos exposure induces the generation of reactive oxygen species, and 8-isoprostane is involved in experimental asbestos-related lung toxicity. This oxidative stress marker was measured in exhaled breath condensate (EBC) in 92 former asbestos workers with mean age 68.8 +/- 1.7 yr and mean duration of asbestos exposure 24.1 +/- 2.0 yr. The control group had 46 subjects with mean age 65.2 +/- 3.3 yr. The mean level of 8-isoprostane, analyzed by liquid chromatography -electrospray ionization- mass spectrometry, was higher in asbestos-exposed subjects (69.5 +/- 6.6 pg/ml, p=0.0001) compared with the control group, where the concentration was 47.0 +/- 7.8 pg/ml. The results presented support the hypothesis that oxidative stress due to asbestos is the main cause of increased 8-isoprostane in EBC. Measurement of 8-isoprostane in EBC is a promising non-invasive means for assessing the activity of asbestos-induced diseases.
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收藏
页码：484 / 489
页数：6
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