Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

被引：122

作者：

Hamilton, Eileen P. ^{[1
]}

Kapusta, Aurelie ^{[2
]}

Huvos, Piroska E. ^{[3
]}

Bidwell, Shelby L. ^{[4
]}

Zafar, Nikhat ^{[4
]}

Tang, Haibao ^{[4
]}

Hadjithomas, Michalis ^{[4
]}

Krishnakumar, Vivek ^{[4
]}

Badger, Jonathan H. ^{[4
]}

Caler, Elisabet V. ^{[4
]}

Russ, Carsten ^{[5
]}

Zeng, Qiandong ^{[5
]}

Fan, Lin ^{[5
]}

Levin, Joshua Z. ^{[5
]}

Shea, Terrance ^{[5
]}

Young, Sarah K. ^{[5
]}

Hegarty, Ryan ^{[5
]}

Daza, Riza ^{[5
]}

Gujja, Sharvari ^{[5
]}

Wortman, Jennifer R. ^{[5
]}

Birren, Bruce W. ^{[5
]}

Nusbaum, Chad ^{[5
]}

Thomas, Jainy ^{[2
]}

Carey, Clayton M. ^{[2
]}

Pritham, Ellen J. ^{[2
]}

Feschotte, Cedric ^{[2
]}

Noto, Tomoko ^{[6
]}

Mochizuki, Kazufumi ^{[6
]}

Papazyan, Romeo ^{[7
]}

Taverna, Sean D. ^{[7
]}

Dear, Paul H. ^{[8
]}

Cassidy-Hanley, Donna M. ^{[9
]}

Xiong, Jie ^{[10
]}

Miao, Wei ^{[10
]}

Orias, Eduardo ^{[1
]}

Coyne, Robert S. ^{[4
]}

机构：

[1] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

[2] Univ Utah, Sch Med, Dept Human Genet, Salt Lake City, UT 84132 USA

[3] Southern Illinois Univ, Biochem & Mol Biol, Carbondale, IL USA

[4] J Craig Venter Inst, Rockville, MD USA

[5] Eli & Edythe L Broad Inst Harvard & MIT, Cambridge, MA USA

[6] Inst Mol Biotechnol, Vienna, Austria

[7] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[8] MRC Lab Mol Biol, Cambridge, England

[9] Cornell Univ, Dept Microbiol & Immunol, Ithaca, NY USA

[10] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

来源：

ELIFE | 2016年 / 5卷

基金：

奥地利科学基金会; 美国国家卫生研究院; 美国国家科学基金会;

关键词：

CHROMOSOME BREAKAGE SITES; PROGRAMMED DNA ELIMINATION; RIBOSOMAL-RNA GENE; TRANSPOSABLE ELEMENTS; WIDE CHARACTERIZATION; CHROMATIN DIMINUTION; NUCLEOTIDE-SEQUENCE; LINE MICRONUCLEUS; INVERTED REPEATS; MACRONUCLEAR DNA;

D O I：

10.7554/eLife.19090

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymenas germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.

引用

页数：46

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