Carriage of virulence factors and molecular characteristics of Staphylococcus aureus isolates associated with bloodstream, and skin and soft tissue infections in children

被引:9
作者
Li, T.
Yu, X. [2 ]
Xie, J. [1 ]
Xu, Y. [1 ]
Shang, Y. [3 ]
Liu, Y. [1 ]
Huang, X. [1 ]
Qin, Z. [4 ,5 ]
Parsons, C. [4 ,5 ]
Hu, L. [6 ]
Salgado, C. [7 ]
Wang, L. [1 ]
Yu, F. [3 ]
机构
[1] Wenzhou Med Coll, Affiliated Hosp 1, Dept Resp Med, Wenzhou, Peoples R China
[2] Jiangxi Prov Children Hosp, Dept Lab Med, Nanchang, Peoples R China
[3] Wenzhou Med Coll, Affiliated Hosp 1, Dept Clin Lab, Wenzhou, Peoples R China
[4] Louisiana State Univ, Hlth Sci Ctr, Dept Med, New Orleans, LA USA
[5] Louisiana State Univ, Hlth Sci Ctr, Dept Microbiol, New Orleans, LA USA
[6] Nanchang Univ, Affiliated Hosp 2, Dept Clin Lab, Nanchang, Peoples R China
[7] Med Univ S Carolina, Dept Med, Hollings Canc Ctr, Div Infect Dis, Charleston, SC 29425 USA
关键词
Bloodstream infection; molecular typing; skin and soft tissue infection; Staphylococcus aureus; virulence genes; METHICILLIN-RESISTANT; ASSIGNMENT; PREVALENCE; SEPSIS; GENES; MEC;
D O I
10.1017/S0950268812002919
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
We investigated the virulence gene carriage and molecular type characteristics of Staphylococcus aureus isolates from bloodstream infections (BSIs) and skin and soft tissue infections (SSTIs) in children. A total of 71 isolates, 16 of which were methicillin-resistant S. aureus (MRSA), were investigated by PCR for virulence-associated gene profiles, sequence type and spa type. This revealed that 76.7% and 53.7% of the SSTI and BSI isolates, respectively, exhibited simultaneous carriage of >= 10 virulence genes. Compared to BSI isolates, carriage rates for hla, hlb, cna, clfA, seb, sec and pvl genes were significantly higher in SSTI isolates. By contrast, carriage of eta, etb and sea was significantly higher for BSI isolates. Thirty-four sequence types (STs) and 36 spa types were identified in the 71 isolates and included 14 novel STs and four novel spa types. ST59-MRSA-IV/V-t437 was the most common clone in the MRSA isolates. We concluded that virulence determinants are widely distributed in isolates of S. aureus strains from children with BSIs and SSTIs, with an unexpectedly high rate in SSTI isolates. Future profiling of S. aureus virulence determinants may allow the prediction of severity and outcome for children with these infections.
引用
收藏
页码:2158 / 2162
页数:5
相关论文
共 50 条
  • [41] Clinical characteristics, virulence factors and molecular typing of methicillin-resistant Staphylococcus aureus infections in Shenzhen City, China
    Hu, L.
    Li, Y.
    Lu, Y.
    Klena, J. D.
    Qiu, Y.
    Lin, Y.
    Jiang, M.
    Shi, X.
    Chen, L.
    Liu, X.
    Ma, H.
    Cheng, J.
    Wu, S.
    Kan, B.
    Hu, Q.
    EPIDEMIOLOGY AND INFECTION, 2016, 144 (14) : 3037 - 3045
  • [42] Staphylococcus aureus Causing Skin and Soft Tissue Infections in Companion Animals: Antimicrobial Resistance Profiles and Clonal Lineages
    Costa, Sofia Santos
    Ribeiro, Rute
    Serrano, Maria
    Oliveira, Ketlyn
    Ferreira, Carolina
    Leal, Marta
    Pomba, Constanca
    Couto, Isabel
    ANTIBIOTICS-BASEL, 2022, 11 (05):
  • [43] A review of community-associated methicillin-resistant Staphylococcus aureus skin and soft tissue infections
    Gorwitz, Rachel J.
    PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2008, 27 (01) : 1 - 7
  • [44] Antibiotic Resistance Pattern of Staphylococcus Aureus Causing Skin and Soft Tissue Infections
    Jebadass, Jasmine Vinshia
    Padmavathy, Kesavaram
    Malaiyan, Jeevan
    Gnanadesikan, Sumathi
    INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH, 2021, : 24 - 27
  • [45] Staphylococcus aureus skin and soft-tissue infections at a tertiary hospital in Botswana
    Truong, Hong
    Shah, Samir S.
    Ludmir, Jonathan
    Tawanana, Ephraim O.
    Bafana, Margaret
    Wood, Sarah M.
    Moffat, Howard
    Steenhoff, Andrew P.
    SAMJ SOUTH AFRICAN MEDICAL JOURNAL, 2011, 101 (06): : 413 - 416
  • [46] Low Frequency of Staphylococcus Aureus in Lower Extremity Skin and Soft Tissue Infections
    Tanveer, Farah
    Bhargava, Ashish
    Riederer, Kathleen
    Johnson, Leonard B.
    Khatib, Riad
    AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 2018, 356 (06) : 528 - 530
  • [47] Skin and soft tissue infections due to Panton-Valentine leukocidin producing Staphylococcus aureus
    Gros, C.
    Yazdanpanah, Y.
    Vachet, A.
    Roussel-Delvallez, M.
    Senneville, E.
    Lemaire, X.
    MEDECINE ET MALADIES INFECTIEUSES, 2012, 42 (10): : 488 - 494
  • [48] Clonal diversity, virulence-associated genes and antimicrobial resistance profile of Staphylococcus aureus isolates from nasal cavities and soft tissue infections in wild ruminants in Italian Alps
    Luzzago, Camilla
    Locatelli, Clara
    Franco, Alessia
    Scaccabarozzi, Licia
    Gualdi, Valentina
    Vigano, Roberto
    Sironi, Giuseppe
    Besozzi, Martina
    Castiglioni, Bianca
    Lanfranchi, Paolo
    Cremonesi, Paola
    Battisti, Antonio
    VETERINARY MICROBIOLOGY, 2014, 170 (1-2) : 157 - 161
  • [49] Molecular Characteristics and Distribution of Virulence Genes among Staphylococcus aureus Complex Isolates Derived from Vascular Access Infections
    Wong, Min Yi
    Tseng, Yuan-Hsi
    Huang, Tsung-Yu
    Chu, Chishih
    Lin, Bor-Shyh
    Huang, Yao-Kuang
    CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY, 2022, 2022
  • [50] Molecular characteristics of methicillin-resistant Staphylococcus aureus isolated from skin and soft tissue infections collected in the Japanese nationwide surveillance
    Kaku, Norihito
    Ohnishi, Takamitsu
    Matsumoto, Tetsuya
    Watanabe, Shinichi
    Yanagihara, Katsunori
    JOURNAL OF DERMATOLOGY, 2019, 46 (12) : 1205 - 1209