Effect of the Cry1, Cry2 and Vip3 protein combinations on the control ofAnticarsia gemmatalis(Erebidae) andChrysodeixis includens(Noctuidae) Lepidoptera

被引：3

作者：

Figueiredo, Camila Soares ^{[1
]}

Zara, Fernando Jose ^{[2
]}

Desiderio, Janete Apparecida ^{[1
]}

机构：

[1] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, Jaboticabal, SP, Brazil

[2] UNESP, IEAMAR, Aquaculture Ctr CAUNESP, Sao Vicente, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF PEST MANAGEMENT | 2022年 / 68卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Synergism; antagonism; heterologous competition; resistance management; soybean looper; velvetbean caterpillar; SPODOPTERA-FRUGIPERDA MIDGUT; BACILLUS-THURINGIENSIS CRY1; ANTICARSIA-GEMMATALIS; HELICOVERPA-ARMIGERA; BINDING-SITES; LARVAL MIDGUT; NOCTUIDAE; TOXIN; RESISTANCE; SUSCEPTIBILITY;

D O I：

10.1080/09670874.2020.1817617

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The transgenic soybean expressing thecry1Acinsecticidal gene of theBacillus thuringiensisbacterium has been widely adopted in agriculture. This bacterium acts as an efficient control agent of pest insects due to the expression of different proteins with entomopathogenic activity such as Cry and Vip. This study investigates the toxicity and mode of interaction between the Cry1Ab, Cry1Ac, Cry2Aa, Cry2Ab and Vip3Aa proteins againstChrysodeixis includensandAnticarsia gemmatalislarvae. The combination of the Cry1Ac, Cry2Aa and Vip3Aa toxins had a synergistic effect forC. includens, however, only Vip3Aa and Cry2Aa exhibited synergistic interaction againstA. gemmatalis. Cry1Ab and Cry1Ac proteins have been shown to share receptors with the two species tested, however, Cry1Ac does not share binding sites with Cry2Aa, Cry2Ab, and Vip3Aa in both species. Besides helping the resistance management toB. thuringiensisproteins, the combination of Cry and Vip proteins can increase toxicity through synergistic action.

引用

页码：175 / 183

页数：9

共 41 条

[11] Characterization of the resistance to Vip3Aa in Helicoverpa armigera from Australia and the role of midgut processing and receptor binding [J].

Chakroun, Maissa ;

Banyuls, Nuria ;

Walsh, Tom ;

Downes, Sharon ;

James, Bill ;

Ferre, Juan .

SCIENTIFIC REPORTS, 2016, 6

[12] In Vivo and In Vitro Binding of Vip3Aa to Spodoptera frugiperda Midgut and Characterization of Binding Sites by 125I Radiolabeling [J].

Chakroun, Maissa ;

Ferre, Juan .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (20) :6258-6265

[13] Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae) [J].

Davolos, Camila C. ;

Hernandez-Martinez, Patricia ;

Crialesi-Legori, Paula C. B. ;

Desiderio, Janete A. ;

Ferre, Juan ;

Escriche, Baltasar ;

Lemos, Manoel Victor F. .

JOURNAL OF INVERTEBRATE PATHOLOGY, 2015, 127 :32-34

[14] High Susceptibility to Cry1Ac and Low Resistance Allele Frequency Reduce the Risk of Resistance of Helicoverpa armigers to Bt Soybean in Brazil [J].

Dourado, Patrick M. ;

Bacalhau, Fabiana B. ;

Amado, Douglas ;

Carvalho, Renato A. ;

Martinelli, Samuel ;

Head, Graham P. ;

Omoto, Celso .

PLOS ONE, 2016, 11 (08)

[15] Interaction of Bacillus thuringiensis toxins with larval midgut binding sites of Helicoverpa armigera (Lepidoptera: Noctuidae) [J].

Estela, A ;

Escriche, B ;

Ferré, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (03) :1378-1384

[16] Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects [J].

Estruch, JJ ;

Warren, GW ;

Mullins, MA ;

Nye, GJ ;

Craig, JA ;

Koziel, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5389-5394

[17] Biochemistry and genetics of insect resistance to Bacillus thuringiensis [J].

Ferré, J ;

Van Rie, J .

ANNUAL REVIEW OF ENTOMOLOGY, 2002, 47 :501-533

[18] Characterization of the vip3A gene and toxicity of Vip3Aa50 protein to fall armyworm and velvetbean caterpillar [J].

Figueiredo, Camila Soares ;

Marucci, Suzana Cristina ;

Dozzi Tezza, Renata Izabel ;

Franco Lemos, Manoel Victor ;

Desiderio, Janete Apparecida .

PESQUISA AGROPECUARIA BRASILEIRA, 2013, 48 (09) :1220-1227

[19]

FINNEY D J, 1971, P333

[20]

Gomis-Cebolla J, 2017, J INVERTEBR PATHOL, V142, P60, DOI [10.1016/j.jip.2016.10.001, 10.1016/j.jip.2016.09.006]

← 1 2 3 4 5 →